Recombinant Human PPM1D His Protein

Novus Biologicals | Catalog # NBP2-51998

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all PPM1D Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 98-375 of Human PPM1D

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MVAFFAVCDG HGGREAAQFA REHFWGFIKK QKGFTSSEPA KVCAAIRKGF LACHLAMWKK LAEWPKTMTG LPSTSGTTAS VVIIRGMKMY VAHVGDSGVV LGIQDDPKDD FVRAVEVTQD HKPELPKERE RIEGLGGSVM NKSGVNRVVW KRPRLTHNGP VRRSTVIDQI PFLAVARALG DLWSYDFFSG EFVVSPEPDT SVHTLDPQKH KYIILGSDGL WNMIPPQDAI SMCQDQEEKK YLMGEHGQSC AKMLVNRALG RWRQRMLRAD NTSAIVICI

Purity

>85%, by SDS-PAGE

Predicted Molecular Mass

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human PPM1D His Protein

SDS-PAGE: Recombinant Human PPM1D His Protein [NBP2-51998]

SDS-PAGE: Recombinant Human PPM1D His Protein [NBP2-51998]

SDS-Page: Recombinant Human PPM1D Protein [NBP2-51998] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain

Formulation, Preparation, and Storage

NBP2-51998
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol
Preservative No Preservative
Concentration 0.5 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: PPM1D

PPM1D is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development.

Long Name

Protein Phosphatase, Mg2+/Mn2+ Dependent, 1D

Alternate Names

PP2C-delta, Wip1

Gene Symbol

PPM1D

Additional PPM1D Products

Product Documents for Recombinant Human PPM1D His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human PPM1D His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics
Phosphatases and Regulators

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