Amino Acid Sequence: LPVCSGGDCQ TPLPELFDRV VMLSHYIHTL YTDMFIEFDK QYVQDREFIA KAINDCPTSS LATPEDKEQA QKVPPEVLLN LILSLVHSWN DPLFQLITGL GGIHEAPDAI ISRAKEIEEQ NKRLLEGIEK IISQAYPEAK GNEIYLVWSQ LPSLQGVDEE SKDLAFYNNI RCLRRDSHKV DNYLKFLRCQ IVHKNNC

Purity

> 98 % pure by SDS-PAGE and HPLC

Endotoxin Level

Less than 0.1 EU/ug of Prolactin as determined by LAL method.

Predicted Molecular Mass

22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Activity

Prolactin protein is fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using rat Nb2-11 cells is less than 1.0 ng/ml, corresponding to a specific activity of > 1.0 x 10^6 IU/mg.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Rat Prolactin Protein

SDS-PAGE: Recombinant Rat Prolactin Protein [NBP2-35287]

SDS-Page: Rat Prolactin Protein [NBP2-35287]

Formulation, Preparation, and Storage

NBP2-35287

Formulation	Lyophilized from a 0.2 um filtered concentrated solution in PBS, pH 7.4.
Preservative	No Preservative
Concentration	Lyoph
Reconstitution	Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.
Format	Carrier-Free
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Prolactin

Prolactin, also named PRL and lactotrope is a peptide hormone, encoded by the PRL gene. The structure of prolactin is similar to that of growth hormone and placental lactogen. There are mainly three different forms of prolactin in regard to size: little prolactin, big prolactin and big big prolactin. Little prolactin is the predominant form. Although often associated with human milk production, prolactin plays a wide range of other roles in both humans and other verterbrates. Rat prolactin shares 77 % ~ 90 % a.a. sequence identity with human and murine prolactin. Recombinant rat prolactin is a 22.6 kDa globular protein containing 197 amino acid residues.

Alternate Names

PRL

Gene Symbol

PRL

UniProt

P01237 (Rat)

Additional Prolactin Products

Product Documents for Recombinant Rat Prolactin Protein

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Certificate of Analysis

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Product Specific Notices for Recombinant Rat Prolactin Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Recombinant Rat Prolactin Protein

Novus Biologicals | Catalog # NBP2-35287

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