Propidium iodide

Catalog # Availability Size / Price Qty
Propidium iodide | CAS No. 25535-16-4 | Fluorescent DNA Stains
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Description: Red-fluorescent DNA stain; membrane impermeant to live cells. Pi staining differentiates live and dead cells
Alternative Names: PI

Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide

Purity: ≥95%

Product Details
Reviews (2)

Biological Activity

Propidium iodide is a red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.

Optical Data for Propidium iodide

λabs 535 nm
λem 617 nm
Application Flow Cytometry

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Technical Data

Soluble to 5 mM in water with sonication
Store at -20°C

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

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Average Rating: 4.5 (Based on 2 Reviews)

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Good Product
By Rohit Sharma on 08/17/2020
Application: Species: Human

PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.

Propidium dye for intracellular fluorescent dye.
By Anonymous on 03/13/2018
Application: Species: Mouse

Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.

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