Propidium iodide
Chemical Name: 3,8-Diamino-5-[3-(diethylmethylammonio)propyl]-6-phenylphenanthridinium diiodide
Purity: ≥95%
Biological Activity
Propidium iodide is a red-fluorescent DNA stain. Membrane impermeant to live cells. Propidium iodide staining differentiates between live and dead cells in cell populations.Optical Data for Propidium iodide
| λabs | 535 nm |
|---|---|
| λem | 617 nm |
| Application | Flow Cytometry |
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Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
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SIRT4 protein suppresses tumor formation in genetic models of Myc-induced B cell lymphoma.
Jeong et al.
J.Biol.Chem., 2013;289:4135 -
p53-dependent and p53-independent anticancer effects of different histone deacetylase inhibitors.
Sonnemann et al.
Br.J.Cancer, 2014;110:656 -
Serine hydrolase inhibitors block necrotic cell death by preventing calcium overload of the mitochondria and permeability transition pore formation.
Yun et al.
J.Biol.Chem., 2013;289:1491
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PI was used in the cell cycle analysis of Jurkat cells. The cell pellet of the treated cells was resuspended in 200 μL propidium iodide (PI) solution containing 50 μg/mL PI, 0.1 mg/mL RNase A, and 0.05% Triton X-100 (v/v) (prepared in 1X PBS buffer). The cells were incubated for 30 min. in the dark at room temperature and by agitating every 10 mins. After incubation, the samples were analyzed using the flow cytometer.
Propidium Iodide was used as an intracellular fluorescent dye. Picture is of the glass electrode with a 5mM solution. 250uM was also tried but had poor visibility.
