Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
11172-VS-050
Recombinant Cynomolgus Monkey/Rhesus Macaque VSIG8 Fc Chimera Protein Bioactivity.
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Recombinant Cynomolgus/Rhesus VSIG8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 or IFN-gamma secretion by human T cells. The ED50 for this effect 0.500-10.0 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived VSIG8 protein
Cynomolgus Monkey/Rhesus Macaque VSIG8
(Val24-Gly265)
Accession # XP_028702448.1
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val24
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
54 kDa
SDS-PAGE
56-63 kDa, under reducing conditions.

Product Datasheets

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11172-VS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11172-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity View Larger

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human T cells. The ED50 for this effect 0.500-10.0 μg/mL.

SDS-PAGE View Larger

2 μg/lane of Recombinant Cynomolgus Monkey/Rhesus Macaque VSIG8 Fc Chimera Protein (Catalog # 11172-VS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 56-63 kDa and 110-130 kDa, respectively.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Based on its similarity with human VSIG8, cynomolgus VSIG8 is predicted to consist of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, cynomolgus VSIG8 shares 99% and 100% aa identity with human and rhesus VSIG8, respectively.  VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References
  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.
Long Name
V-Set and Immunoglobulin Domain Containing 8
Entrez Gene IDs
391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)
Alternate Names
V-set and immunoglobulin domain containing 8; VSIG8

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