Recombinant Human ACP6 Protein, CF

R&D Systems | Catalog # 7766-AP

R&D Systems
100% Guarantee

Key Product Details

  • R&D Systems CHO-derived Recombinant Human ACP6 Protein (7766-AP)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

BAA91310

Applications

Enzyme Activity
View all ACP6 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human ACP6 protein
Glu33-Glu428, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu33

Predicted Molecular Mass

46 kDa

SDS-PAGE

43-45 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >1800 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation, and Storage

7766-AP
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: ACP6

Lysophosphatidic Acid Phosphatase Type 6 (ACP6) is a membrane-bound protein that belongs to the Histidine Acid Phosphatase Family. ACP6 is expressed in kidney, heart, small intestine, muscle, liver, prostate, testis, ovary, and colon tissues (1, 2). Its function is to hydrolyze lysophosphatidic acid (LPA) to form monoacylglycerol. LPA is one of the simplest of all known phospholipids which has a variety of biological functions including cell proliferation, migration, and survival (3). LPA is found at high levels in serum but also present in saliva, follicular fluid, seminal plasma and malignant effusions and is implicated in many cancers including ovarian, breast and prostate (3-5).

References

  1. Hiroyama, M. and T. Takenawa (1999) J. Biol. Chem. 274:29172.
  2. Takayama I. et al. (2002) Gut 50:790.
  3. Mills, G. and W. Moolenaar (2003) Nature 3:582.
  4. Xu, Y. et al. (1995) Biochem J. 309:933.
  5. Xie, Y. et al. (2002) J. Biol. Chem. 277:32516.

Long Name

Lysophosphatidic Acid Phosphatase 6

Alternate Names

ACPL1, LPAP, PACPL1

Entrez Gene IDs

51205 (Human); 66659 (Mouse); 295305 (Rat)

Gene Symbol

ACP6

UniProt

BAA91310

Additional ACP6 Products

Product Documents for Recombinant Human ACP6 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human ACP6 Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd. Triton is a registered trademark of Union Carbide Corp.

For research use only

Customer Reviews for Recombinant Human ACP6 Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human ACP6 Protein, CF and earn rewards!

Have you used Recombinant Human ACP6 Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for Recombinant Human ACP6 Protein, CF (7766-AP):

Materials
  • Assay Buffer: 50 mM Sodium acetate, 0.05% Triton® X-100, pH 5.0
  • Recombinant Human ACP6 (rhACP6) (Catalog # 7766-AP)
  • Substrate: p-Nitrophenyl phosphate (Sigma, Catalog # N2765), 10 mM stock in deionized water
  • NaOH, 0.2 M in deionized water
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate Reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhACP6 to 0.8 µg/mL in Assay buffer.
  2. Dilute Substrate to 2 mM in Assay buffer.
  3. In a plate, load 50 µL of 0.8 µg/mL rhACP6, and start the reaction by adding 50 µL of 2 mM Substrate. Include a Substrate Blank containing 50 µL of Assay buffer and 50 µL of 2 mM Substrate.
  4. Incubate plate at room temperature for 30 minutes in the dark.
  5. Add 100 µL of 0.2 M NaOH to all wells to stop the reaction and develop the color.
  6. Read plate at 410 nm (absorbance) in endpoint mode.
  7. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Abs* (OD) x Conversion Factor** (pmol/OD)
Incubation time (min) x amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard p-Nitrophenol (Sigma, Catalog # 241326).

Per Well:
  • rhACP6: 0.04 µg
  • pNPP: 0.5 mM

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes