The human XPNPEP1 gene encodes aminopeptidase P1 (APP1), which is also known as X-prolyl aminopeptidase with gene aliases of SAMP, XPNPEP, XPNPEPL, and XPNPEPL1 (1‑3). It is a member of the M24 family of metalloproteases, which also contains methionine aminopeptidases, X-Pro dipeptidase, aminopeptidase P2, aminopeptidase P homolog, proliferation-associated protein 1, and suppressor of Ty homolog or chromatin-specific transcription elongation factor large subunit (4). It is a soluble enzyme, in contrast to the GPI-anchored APP2 encoded by XPNPEP2 (5). Human APP1 is widely expressed (3). The purified rhAPP1 is an active aminopeptidase, removing a N‑terminal amino acid from a peptide that contains a Pro residue at the second position. The amino acid sequence of human APP1 is 99%, 97%, 95%, 74% and 73% identical to that of canine, bovine, mouse/rat, Xenopus and zebrafish, respectively.
Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
R&D Systems | Catalog # 2970-ZN
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Key Product Details
- R&D Systems E. coli-derived Recombinant Human Aminopeptidase P1/XPNPEP1 Protein (2970-ZN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
E. coli
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
E. coli-derived human Aminopeptidase P1/XPNPEP1 protein
Met1-His623 & Pro2-His623, both with a C-terminal 6-His tag
Met1-His623 & Pro2-His623, both with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met1 & Pro2
Predicted Molecular Mass
71 kDa
SDS-PAGE
71 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, H-Lys(2-Aminobenzoyl)-Pro-Pro-p-Nitroanilide (K(Abz)PP-pNA).
The specific activity is >1,300 pmol/min/µg, as measured under the described conditions.
The specific activity is >1,300 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation, and Storage
2970-ZN
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Aminopeptidase P1/XPNPEP1
References
- Cottrell, G.S. et al. (2000) Biochemistry 39:15121.
- Sprinkle, T.J. et al. (2000) Arch. Biochem. Biophys. 378:51.
- Vanhoof, G. et al. (1997) Cytogenet. Cell Genet. 78:275.
- Turner, A.J. and G.S. Cottrell (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 931 - 933, Elsevier Academic Press, San Diego.
- Simmons, W.H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 934 - 937, Elsevier Academic Press, San Diego.
Alternate Names
APP1, SAMP, XPNPEP1
Entrez Gene IDs
7511 (Human)
Gene Symbol
XPNPEP1
UniProt
Additional Aminopeptidase P1/XPNPEP1 Products
Product Documents for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF
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Protocols
View specific protocols for Recombinant Human Aminopeptidase P1/XPNPEP1 Protein, CF (2970-ZN):
Materials
- Assay Buffer: 50 mM Tris, 250 mM NaCl, 0.5 mM MnCl2, pH 8.0
- Recombinant Human Aminopeptidase P1/XPNPEP1 (rhXPNPEP1) (Catalog # 2970-ZN)
- Substrate: Lys(ABZ)Pro-Pro-PNA (Bachem, Catalog # L-1980), 10 mM stock in deionized water
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhXPNPEP1 to 0.5 ng/µL in Assay Buffer.
- Dilute Substrate to 100 µM in Assay Buffer.
- Load into plate 50 µL of 0.5 ng/µL of rhXPNPEP1 (25 ng/well), and start the reaction by adding 50 µL of 100 µM Substrate. Include a Substrate Blank of 50 μL Assay Buffer and 50 µL of 100 µM Substrate.
- Read at excitation and emission wavelengths of 320 nm and 410 nm (top read), respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
| Specific Activity (pmol/min/µg) | Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard Abz-Gly (Bachem, Catalog # E-2920).
Per Well:- rhXPNPEP1: 0.025 µg
- Substrate: 50 µM