Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF Summary
Ala37-Met941, with a C-terminal 10-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Supplied as a 0.2 μm filtered solution in Tris and NaCl.|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- Assay Buffer: 25 mM Tris, pH 8.0
- Recombinant Human Aminopeptidase PILS/ARTS1 (rhARTS1) (Catalog # 2334-ZN)
- Substrate: Leu-AMC (Bachem, Catalog # I-1240)
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rhARTS1 to 2 µg/mL in Assay Buffer.
- Dilute Substrate to 600 µM in Assay Buffer.
- Load 50 µL of 2 µg/mL rhARTS1 into the plate, and start the reaction by adding 50 µL of 600 µM Substrate. Include a Substrate Blank containing Assay Buffer and Substrate.
- Read at excitation and emission wavelengths of 380 nm and 460 nm (respectively), in kinetic mode for 5 minutes.
- Calculate specific activity:
Specific Activity (pmol/min/µg) =
|Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU)|
|amount of enzyme (µg)|
*Adjusted for Substrate Blank
**Derived using calibration standard 7-amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
- rhARTS1: 0.1 µg
- Substrate: 300 µM
Background: Aminopeptidase PILS/ARTS1
The name of Aminopetidase PILS (Puromycin-Insensitive Leucyl-Specific) describes the two basic properties of this zinc metalloprotease in vitro (1). Also known as ALAP (Adipocyte-derived Leucin AminoPeptidase), type 1 tumor necrosis factor receptor (TNFR) shedding aminopeptidase regulator and ER aminopeptidase ERAP1 or ERAAP, it is encoded by the ARTS1 gene (2-4). Aminopeptidase PILS has been identified to regulate antigen presentation, promote TNFR1 ectodomain shedding and associate with hypertension (2-5).
- Schomburg, L. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 311, Academic Press, San Diego.
- Cui, X. et al. (2002) J. Clin. Invest. 110:515.
- York, I.A. et al. (2002) Nat. Immunol. 3:1177.
- Serwold, T. et al. (2002) Nature 419:480.
- Yamamoto, N. et al. (2002) Hum. Mutat. 19:251.
Citations for Recombinant Human Aminopeptidase PILS/ARTS1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Modulators of hERAP2 discovered by high-throughput screening
Authors: M Laura, G Ronan, LB Vy, G Valentin, CA Omar, C Virgyl, C Piveteau, R Melissa, F Charlotte, W Sandrine, C Julie, DR Julie, B Damien, L Florence, VE Peter, D Benoit, DP Rebecca
European Journal of Medicinal Chemistry, 2020;211(0):113053.
ER-aminopeptidase 1 determines the processing and presentation of an immunotherapy-relevant melanoma epitope
Authors: K Textoris-T, C Cammann, P Henklein, E Topfstedt, F Ebstein, S Henze, J Liepe, F Zhao, D Schadendor, B Dahlmann, W Uckert, A Paschen, M Mishto, U Seifert
Eur. J. Immunol., 2019;0(0):.
Sample Types: Protein
Applications: Enzyme Assay
Sequence-specific alterations of epitope production by HIV protease inhibitors.
Authors: Kourjian, Georgio, Xu, Yang, Mondesire-Crump, Ijah, Shimada, Mariko, Gourdain, Pauline, Le Gall, Sylvie
J Immunol, 2014;192(8):3496-506.
Sample Types: Protein
Applications: Enzyme Assay
CHR-2797: an antiproliferative aminopeptidase inhibitor that leads to amino acid deprivation in human leukemic cells.
Authors: Krige D, Needham LA, Bawden LJ, Flores N, Farmer H, Miles LE, Stone E, Callaghan J, Chandler S, Clark VL, Kirwin-Jones P, Legris V, Owen J, Patel T, Wood S, Box G, Laber D, Odedra R, Wright A, Wood LM, Eccles SA, Bone EA, Ayscough A, Drummond AH
Cancer Res., 2008;68(16):6669-79.
Sample Types: Whole Cells
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