Recombinant Human ASP/C3a desArg Protein

Formulations:
Catalog # Availability Size / Price Qty
9620-C3-025
Recombinant Human ASP/C3a desArg Protein Bioactivity
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Recombinant Human ASP/C3a desArg Protein Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to induce IL-6 secretion by LPS-stimulated human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.4-2.4 μg/mL.
Source
E. coli-derived human ASP/C3a desArg protein
Ser672-Ala747
Accession #
N-terminal Sequence
Analysis
Ser672
Predicted Molecular Mass
9 kDa
SDS-PAGE
9 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9620-C3

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9620-C3/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity View Larger

Recombinant Human ASP/C3a desArg (Catalog # 9620‑C3) induces IL‑6 secretion from LPS stimulated PBMCs. The ED50for this effect is 0.4‑2.4 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

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Background: ASP/C3a desArg

Acylation-stimulating protein (ASP), also known as C3adesArg, is an approximately 14 kDa member of the complement family that plays a role in the regulation of triglyceride synthesis (1-4). The secreted protein is a polypeptide fragment comprising amino acids (aa) 672-747 of the Complement C3 precursor protein (4). The polypeptide results from desargination of the carboxyl terminus of C3a, which is generated from interactions among C3, factor B, and adipsin (4). ASP contains a 36 aa anaphylatoxin-like domain (5). Human ASP shares 68% sequence identity with mouse and rat ASP. Increased levels of ASP are usually associated with insulin resistance, obesity and diabetes. It promotes triacylglycerol synthesis in adipocytes and inflammatory cytokine production in a septic environment (6-8).

References
  1. Cianflone, K. et al. (1989) J. Biol. Chem. 264:426.
  2. Baldo, A. et al. (1993) J. Clin. Invest. 92:1543.
  3. Havel, P. (2002) Curr. Opin. Lipidol. 13:51.
  4. Cianflone, K. et al. (2003) Biochim. Biophys. Acta. 1609:127.
  5. Kolev, M. et al. (2017) Front. Immunol. 8:1.
  6. Murray, I. et al. (1999) Biochem. J. 342:41.
  7. Munkonda, M. N. et al. (2012) PLOS One. 7(10):1.
  8. Takabayashi, T. et al. (1998) JID. 177:1622.
Long Name
Acylation Stimulating Protein
Alternate Names
Acylation Stimulating Protein; ASP; C3a des Arg

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