Recombinant Human Carbonic Anhydrase III Protein, CF

R&D Systems | Catalog # 2185-CA

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Human Carbonic Anhydrase III Protein (2185-CA)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

P07451

Applications

Enzyme Activity
View all Carbonic Anhydrase III/CA3 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Carbonic Anhydrase III/CA3 protein
Ala2-Lys260

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala2

Predicted Molecular Mass

31 kDa

SDS-PAGE

30 kDa, reducing conditions

Activity

Measured by its esterase activity.
The specific activity is >1 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation, and Storage

2185-CA
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 1 mg/mL in sterile 25 mM Tris and 150 mM NaCl, pH 7.5.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carbonic Anhydrase III/CA3

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2 + H2O = HCO3- + H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1).

Topics in a CA meeting (6th International Conference on the CAs, June 20-25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO2 removal and mosquito control in industry. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth (2).

References

  1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
  2. Sly, W.S. and P.Y. Hu (1995) Annu. Rev. Biochem. 64:375.

Alternate Names

CA3

Entrez Gene IDs

761 (Human)

Gene Symbol

CA3

UniProt

P07451

Additional Carbonic Anhydrase III/CA3 Products

Product Documents for Recombinant Human Carbonic Anhydrase III Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Carbonic Anhydrase III Protein, CF

For research use only

Citations for Recombinant Human Carbonic Anhydrase III Protein, CF

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Protocols

View specific protocols for Recombinant Human Carbonic Anhydrase III Protein, CF (2185-CA):

Materials
  • Assay Buffer: 12.5 mM Tris, 75 mM NaCl, pH 7.5
  • Recombinant Human Carbonic Anhydrase III/CA3 (rhCA3) (Catalog # 2185-CA)
  • Substrate: 4-Nitrophenyl Acetate (4-NPA) (Sigma, Catalog # N8130), 100 mM stock in acetone
  • 96-well Clear Plate (Costar, Catalog # 92592)
  • Plate reader (Model: SpectraMax Plus by Molecular Devices) or equivalent
  1. Dilute rhCA3 to 400 ng/µL in Assay Buffer.
  2. Dilute Substrate to 2.0 mM in Assay Buffer.
  3. In a plate load 50 µL of 400 ng/µL rhCA3, and include a Substrate Blank containing 50 µL Assay Buffer.
  4. Start the reaction by adding 50 µL of 2.0 mM Substrate to wells containing rhCA3 and Assay Buffer.
  5. Read at a wavelength of 400 nm (bottom read) in kinetic mode for 5 minutes.
  6. Calculate specific activity:

     Specific Activity (pmol/min/µg) =

 Adjusted Vmax* (OD/min) x Conversion Factor** (pmol/OD)
amount of enzyme (µg)

     *Adjusted for Substrate Blank
     **Derived using calibration standard 4-Nitrophenol (Sigma, Catalog # 241326).

Per Well:
  • rhCA3: 20 µg
  • Substrate: 1.0 mM

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