Recombinant Human CRELD1 Protein, CF

R&D Systems | Catalog # 3128-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Human CRELD1 Protein (3128-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q96HD1

Applications

Bioactivity
View all CRELD1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CRELD1 protein
Gln30-Glu362, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln30 predicted, sequencing might be blocked

Predicted Molecular Mass

36.8 kDa

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is 0.3-1.2 μg/mL.

Formulation, Preparation, and Storage

3128-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRELD1

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa (1). Alternative splicing of human CRELD1 generates an additional isoform with a substitution that encompasses the transmembrane segments and the C‑terminal ECD (1). CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro‑apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha 1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). R&D Systems in‑house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225.
  3. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169.
  4. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Alternate Names

AVSD2, CIRRIN

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Gene Symbol

CRELD1

UniProt

Q96HD1

Additional CRELD1 Products

Product Documents for Recombinant Human CRELD1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human CRELD1 Protein, CF

For research use only

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