Recombinant Human ELFN2 Protein, CF

R&D Systems | Catalog # 9329-EF

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Key Product Details

  • R&D Systems HEK293-derived Recombinant Human ELFN2 Protein (9329-EF)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

HEK293

Accession Number

Q5R3F8

Applications

Bioactivity
View all ELFN2 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human ELFN2 protein
Asp23-Tyr397, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp23

Predicted Molecular Mass

43 kDa

SDS-PAGE

71-94 kDa, non-reducing conditions

Activity

Measured by its ability to inhibit neurite outgrowth of E16-E18 rat embryonic cortical neurons on a Laminin  alpha 4 coated plate.
Able to significantly inhibit neurite outgrowth when immobilized as a 3 μL droplet containing 300 ng of protein.

Formulation, Preparation, and Storage

9329-EF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ELFN2

ELFN2 (Extracellular leucine-rich repeat and fibronectin type III domain-containing protein 2), also known as protein phosphatase 1 regulatory subunit 29, is a member of the extracellular Leucine-Rich Repeat superfamily (1). Expressed mainly in the nervous system, ELFN2 is a transmembrane protein that inhibits the activity of protein phosphatase 1 (PP1) complexes (2). Mature human ELFN2 consists of a 375 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 402 aa cytoplasmic tail. The ECD includes one fibronectin type-III domain, five leucine-rich repeats (LRR) and one LRR C-terminal (LRRCT) domain. Human ELFN2 shares 98% aa sequence identity with mouse and rat ELFN2. Expression of ELFN2 is quite restricted to the nervous system in which it is broadly expressed in the cortex in presumed glutamatergic neurons, in hippocampal pyramidal and granule cells, and in the striatum. These patterns are maintained in adults. Given the functions and discrete patterns of many known LRR family proteins it has been proposed that ELFN2 could provide the requisite specificity of cellular interactions to mediate a large number of selective connectivity decisions involved in neurite outgrowth, axon guidance, fasciculation, and synapse formation (1). Mutations of ELFN2 have been reported in pancreatic cancer radiation resistance (3) and stress fracture (4).

References

  1. Dolan J. et al. (2007) BMC Genomics 8:320.
  2. Hendrickx, A. et al. (2009) Chem. Biol. 16:365.
  3. Friedman E. et al. (2014) Genet Res (Camb) 96:e004.
  4. Souchek L.L. et al. (2014) British Journal of Cancer 111:1139.

Long Name

Extracellular Leucine-Rich Repeat And Fibronectin Type III Domain Containing 2

Alternate Names

LRRC62, PPP1R29

Entrez Gene IDs

114794 (Human); 207393 (Mouse)

Gene Symbol

ELFN2

UniProt

Q5R3F8

Additional ELFN2 Products

Product Documents for Recombinant Human ELFN2 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human ELFN2 Protein, CF

For research use only

Related Research Areas

Phosphatases and Regulators

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