Recombinant Human GABARAP Agarose Protein, CF

R&D Systems | Catalog # UL-415

R&D Systems
Discontinued Product
UL-415 has been discontinued. View all GABARAP products.

Key Product Details

Source

E. coli

Accession Number

NP_009209

Conjugate

Agarose

Applications

Enzyme Activity
View all GABARAP Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human GABARAP protein

Activity

Apg8p1 (GABARAP) Agarose is ideal for the enrichment of known Apg8p1-interacting proteins as well as the discovery of novel Apg8p1-interacting proteins. We recommend equilibrating the resin by washing with 5-10 mL of your desired aqueous buffer.

Formulation, Preparation, and Storage

UL-415
Formulation

0.5 ml of GABARAP agarose is supplied in a 1 ml total volume of 100 mM Hepes pH 7.5, 500 mM NaCl, 0.5mM NaN3.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Do not freeze.
  • 3 months from date of receipt, 2 to 8 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: GABARAP

gamma -Aminobutyric Acid Receptor-associated Protein (GABARAP), also known as Apg8p1, is 117 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the GABARAP subfamily of the Autophagy-related 8 (Atg8) family of proteins (1). The GABARAP subfamily also includes GABARAPL1. GABARAP/Apg8p1 has 100% aa sequence identity with its mouse and rat orthologs and is orthologous to yeast Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. GABARAP/Apg8p1 was first described for its putative involvement GABA A Receptor trafficking (2,3). However, it is best known for its role in autophagy (1,4). GABARAP/Apg8p1 covalently attaches to phosphatidylethanolamine (PE) in the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome maturation (4,5). It may also be involved in cargo recruitment to autophagosomes (1).

References

  1. Shpilka, T. et al. (2011) Genome Biol. 12:226.
  2. Wang, H. et al. (1999) Nature 397:69.
  3. Leil, T.A. et al. (2004) J. Neurosci. 24:11429.
  4. Weidberg, H. et al. (2010) EMBO J. 29:1792.
  5. Weidberg, H. et al. (2011) Ann. Rev. Biochem. 80:125.

Long Name

gamma-Aminobutyric Acid Type A Receptor Associated Protein

Alternate Names

Apg8p1, ATG8A, FLC3B, MM46

Entrez Gene IDs

11337 (Human); 56486 (Mouse); 58974 (Rat)

Gene Symbol

GABARAP

UniProt

NP_009209 (Human)

Additional GABARAP Products

Product Documents for Recombinant Human GABARAP Agarose Protein, CF

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Product Specific Notices for Recombinant Human GABARAP Agarose Protein, CF

For research use only

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