Recombinant Human GST-AMSH/STAMBP Protein, CF

R&D Systems | Catalog # E-549

R&D Systems
Discontinued Product
E-549 has been discontinued. View all AMSH/STAMBP products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

O95630

Applications

Bioactivity
View all AMSH/STAMBP Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH/STAMBP protein
Contains an N-terminal GST (glutathione S-transferase) tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

75 kDa

Activity

Recombinant Human GST-AMSH is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-AMSH concentration of 100-500 nM.  GST-AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM.

Formulation, Preparation, and Storage

E-549
Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 10% Glycerol, 150 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: AMSH/STAMBP

AMSH (also known as STAM Binding Protein) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). This enzyme functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. AMSH cleaves K63-linked but not K48-linked polyubiquitin chains, and the in vitro activity of AMSH is greatly increased in the presence of STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.

References

  1. Agromayor, M. and Martin-Serrano, J. (2006) J. Biol. Chem 281:23083
  2. Kim, M.S., et al (2006) Biochem. Biophys. Res Comm. 351:612
  3. McCullough, J., et al (2006) Current Biology 16:160
  4. Sato, Y., et al (2008) Nature. 455:358

Long Name

STAM Binding Protein

Alternate Names

AMSH, STAMBP

Entrez Gene IDs

10617 (Human); 70527 (Mouse); 171565 (Rat)

Gene Symbol

STAMBP

UniProt

O95630

Additional AMSH/STAMBP Products

Product Documents for Recombinant Human GST-AMSH/STAMBP Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GST-AMSH/STAMBP Protein, CF

For research use only

Citations for Recombinant Human GST-AMSH/STAMBP Protein, CF

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