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Recombinant Human GST-AMSH/STAMBP Protein, CF

Catalog #: E-549
2 Citations Datasheet / COA / SDS

Discontinued Product

E-549 has been discontinued.
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Recombinant Human GST-AMSH/STAMBP Protein, CF Summary

Product Specifications

Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
Recombinant Human GST-AMSH is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-AMSH concentration of 100-500 nM.  GST-AMSH activity is partially or fully inhibited by DTT at concentrations above 1 mM.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH/STAMBP protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #
O95630
Predicted Molecular Mass
75 kDa

Product Datasheets

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E-549

Product Datasheet
COA
SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-549

Formulation

X mg/ml (X μM) in 50 mM HEPES pH 8.0, 10% Glycerol, 150 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: AMSH/STAMBP

AMSH (also known as STAM Binding Protein) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). This enzyme functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. AMSH cleaves K63-linked but not K48-linked polyubiquitin chains, and the in vitro activity of AMSH is greatly increased in the presence of STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.

References
  1. Agromayor, M. and Martin-Serrano, J. (2006) J. Biol. Chem 281:23083
  2. Kim, M.S., et al (2006) Biochem. Biophys. Res Comm. 351:612
  3. McCullough, J., et al (2006) Current Biology 16:160
  4. Sato, Y., et al (2008) Nature. 455:358
Long Name
STAM Binding Protein
Entrez Gene IDs
10617 (Human); 70527 (Mouse); 171565 (Rat)
Alternate Names
AMSH; AMSHAssociated molecule with the SH3 domain of STAM; EC 3.1.2.15; EC 3.4.19.-; Endosome-associated ubiquitin isopeptidase; MGC126516; MGC126518; STAM binding protein; STAM-binding protein; STAMBP

Citations for Recombinant Human GST-AMSH/STAMBP Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Site-specific ubiquitination of MLKL targets it to endosomes and targets Listeria and Yersinia to the lysosomes
    Authors: S Yoon, K Bogdanov, D Wallach
    Cell Death and Differentiation, 2022-01-09;0(0):.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  2. Structural and functional characterization of ubiquitin variant inhibitors for the JAMM-family deubiquitinases STAMBP and STAMBPL1
    Authors: Y Guo, Q Liu, E Mallette, C Caba, F Hou, J Fux, G LaPlante, A Dong, Q Zhang, H Zheng, Y Tong, W Zhang
    The Journal of Biological Chemistry, 2021-08-21;0(0):101107.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay

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