Recombinant Human GST-UbcH5b/UBE2D2 Protein, CF

R&D Systems | Catalog # E2-620

R&D Systems
Discontinued Product
E2-620 has been discontinued. View all UbcH5b/UBE2D2 products.

Key Product Details

Source

E. coli

Accession Number

P51669

Applications

Enzyme Activity
View all UbcH5b/UBE2D2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human UbcH5b/UBE2D2 protein
Contains an N-terminal GST (glutathione S-transferase) tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

43 kDa

Activity

Recombinant Human GST-UbcH5b is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human GST-UbcH5b concentration of 0.1-1 μM.

Reviewed Applications

Read 2 reviews rated 4 using E2-620 in the following applications:

Formulation, Preparation, and Storage

E2-620
Formulation X mg/ml (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: UbcH5b/UBE2D2

Ubiquitin-conjugating Enzyme H5b (UbcH5b), also known as Ubiquitin-conjugating Enzyme E2D 2 (UBE2D2), is a widely expressed member of the Ubiquitin-conjugating (E2) enzyme family (1). The protein has a predicted molecular weight of 16.5 kDa. UbcH5b/UBE2D2 localizes to both the nucleus and the cytoplasm (2). The human protein shares 100% and 92% amino acid sequence identity with the mouse and rat orthologs, respectively. This enzyme has an E2 catalytic core domain that contains an active site cysteine residue that is required for the formation of a thioester bond with Ubiquitin (3). UbcH5b/UBE2D2 is capable of mediating the formation of Ubiquitin chains linked through Lys11, Lys48, or Lys63 (4). Working with the SCF(Fbxw2) and MDM2/HDM2 Ubiquitin ligases (E3s), UbcH5b/UBE2D2 mediates the ubiquitination and degradation of the transcription factors GCM1 and p53, respectively (5-7). Along with UBE2N/Ubc13, UbcH5b/UBE2D2 may have a role in the endocytosis and endolysosomal degradation of MHC class I molecules (8). Non-proteolytic ubiquitination of TRIM5-alpha by UbcH5b/UBE2D2 has been reported to block HIV reverse transcription (9). Pathologically, UBE2D family members may be critical targets of cadmium during cadmium-induced renal toxicity (10). Additionally, overexpression of UbcH5b/UBE2D2 has been linked to inflammatory bowel disease (11). This protein has an N-terminal GST-tag.

References

  1. Jensen, J.P. et al. (1995) J. Biol. Chem. 270:30408.
  2. Umebayashi, K. et al. (2008) Mol. Biol. Cell 19:3454.
  3. Hershko, A. et al. (1983) J. Biol. Chem. 258:8206.
  4. Ordureau, A. et al. (2008) Biochem. J. 409:43.
  5. Chiang, M.H. et al. (2008) Biol. Reprod. 79:914.
  6. Saville, M.K. et al. (2004) J. Biol. Chem. 279:42169.
  7. Iyappan, S. et al. (2010) J. Biol. Chem. 285:33065.
  8. Duncan, L.M. et al. (2006) EMBO J. 25:1635.
  9. Yamauchi, K. et al. (2008) FEBS J. 275:1540.
  10. Tokumoto, M. et al. (2011) J. Toxicol. Sci. 36:191.
  11. Hetzenecker, A.M. et al. (2012) Digestion 86:34.

Alternate Names

PUBC1, UBC5B, UBE2D2

Entrez Gene IDs

7322 (Human); 56550 (Mouse); 641452 (Rat)

Gene Symbol

UBE2D2

UniProt

P51669

Additional UbcH5b/UBE2D2 Products

Product Documents for Recombinant Human GST-UbcH5b/UBE2D2 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human GST-UbcH5b/UBE2D2 Protein, CF

For research use only

Customer Reviews for Recombinant Human GST-UbcH5b/UBE2D2 Protein, CF (2)

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  • Name: Jignesh Ganeshbhai Patel
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 09/10/2018

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail