Recombinant Human His6-Miro1/Rhot1 Protein, CF

Catalog # Availability Size / Price Qty
SP-490-100
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human His6-Miro1/Rhot1 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Reaction Conditions will be optimized for each specific application.  In a 50 μl reaction containing 1 μM activated Parkin E3 ligase (E3-160 or E3-162), 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.
Source
E. coli-derived human Miro1/Rhot1 protein
Contains an N-terminal 6-His tag, Ile186 - Thr591
Accession #
Predicted Molecular Mass
50 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-490

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Miro1/Rhot1

Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa.  Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules.  A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria.  This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.

References
  1. Birsa, N. et al. (2014) J. Biol. Chem.  289: 14569
  2. Kazlauskaite, A. et al. (2014) Open Biol.  4: 130213
  3. Klosowiak, J.L. et al. (2016) Sci. Rep.  6: 33019
  4. MacAskill, A.F. et al. (2009)  Neuron  61: 541
  5. Ordureau, A. et al. (2015)  Proc. Natl. Acad. Sci.  112: 637
Long Name
Mitochondrial Rho (MIRO) GTPase 1
Entrez Gene IDs
55288 (Human)
Alternate Names
ARHT1; EC 3.6.5; EC 3.6.5.-; FLJ11040; FLJ12633; hMiro-1; Miro1; MIRO-1mitochondrial Rho GTPase 1; mitochondrial Rho 1; rac-GTP binding protein-like protein; Rac-GTP-binding protein-like protein; Ras homolog gene family member T1; ras homolog gene family, member T1; Rhot1

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