Recombinant Human His6-Miro1/Rhot1 Protein, CF

R&D Systems | Catalog # SP-490

R&D Systems
Discontinued Product
SP-490 has been discontinued. View all Miro1/Rhot1 products.

Key Product Details

Source

E. coli

Accession Number

Q8IXI2

Applications

Bioactivity
View all Miro1/Rhot1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Miro1/Rhot1 protein
Contains an N-terminal 6-His tag, Ile186 - Thr591

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

50 kDa

Activity

Reaction Conditions will be optimized for each specific application.  In a 50 μl reaction containing 1 μM activated Parkin E3 ligase (E3-160 or E3-162), 1 μM His6-Miro1 is fully converted to Ubiquitinated forms in 60 minutes.

Formulation, Preparation, and Storage

SP-490
Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Miro1/Rhot1

Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa.  Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules.  A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria.  This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.

References

  1. Birsa, N. et al. (2014) J. Biol. Chem.  289: 14569
  2. Kazlauskaite, A. et al. (2014) Open Biol.  4: 130213
  3. Klosowiak, J.L. et al. (2016) Sci. Rep.  6: 33019
  4. MacAskill, A.F. et al. (2009)  Neuron  61: 541
  5. Ordureau, A. et al. (2015)  Proc. Natl. Acad. Sci.  112: 637

Long Name

Mitochondrial Rho (MIRO) GTPase 1

Alternate Names

ARHT1, Rhot1

Entrez Gene IDs

55288 (Human)

Gene Symbol

RHOT1

UniProt

Q8IXI2

Additional Miro1/Rhot1 Products

Product Documents for Recombinant Human His6-Miro1/Rhot1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Human His6-Miro1/Rhot1 Protein, CF

For research use only

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