Recombinant Human His6-Miro1/Rhot1 Protein, CF Summary
Contains an N-terminal 6-His tag, Ile186 - Thr591
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 10% (v/v) Glycerol, 2 mM TCEP|
|Shipping||The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Mitochondrial Rho GTPase 1 (also known as Miro1 or Rhot1) is a membrane associated GTPase with a predicted molecular weight of 71 kDa. Miro1 has been reported to directly associate with the KIF5 motor protein, allowing mitochondria to move along microtubules. A number of publications have identifed Miro1 as an in vivo substrate for the E3 Ubiquitin ligase Parkin (encoded by the PARK2 gene), an essential component of the cellular machinery that participates in the removal of damaged mitochondria. This protein is useful as an in vitro substrate for activated recombinant Parkin enzyme.
- Birsa, N. et al. (2014) J. Biol. Chem. 289: 14569
- Kazlauskaite, A. et al. (2014) Open Biol. 4: 130213
- Klosowiak, J.L. et al. (2016) Sci. Rep. 6: 33019
- MacAskill, A.F. et al. (2009) Neuron 61: 541
- Ordureau, A. et al. (2015) Proc. Natl. Acad. Sci. 112: 637
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