Recombinant Human His6-NEDD4 Protein, CF

Catalog # Availability Size / Price Qty
E3-290-050
Product Details
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Recombinant Human His6-NEDD4 Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Activity
Recombinant Human NEDD 4 is a Ubiquitin Ligase (E3) that functions downstream of a Ubiquitin-activating (E1)  enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins.  Reaction conditions will need to be optimized for each specific application.
Source
E. coli-derived human NEDD4 protein
Contains a N-terminal 6-His tag, Leu570 - Asp1319
Accession #
Predicted Molecular Mass
88 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E3-290

Formulation X mg/ml (X μM) in 50 mM HEPES pH 8.0, 100 mM NaCl, 5 mM DTT, 1 mM EDTA
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Reconstitution Calculator

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Background: NEDD4

NEDD4 (Neural precursor cell expressed developmentally down-regulated protein 4) is a Ubiquitin E3 ligase and the prototypical member of an extended family of HECT domain ligases.  Human family members include NEDD4, NEDD4-2, ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDDL1 and NEDDL2.  In addition to the HECT domain, NEDD4 family members also contain a C2 domain and one or more WW domains.  With a predicted molecular weight of 150 kDa, NEDD4 is expressed in most human tissues where it localizes primarily to the perinuclear and other membrane regions.  Evidence suggests that NEDD4 down-regulates multiple membrane proteins (including FGFR1, IGFR1, and multiple EGFR family members) in a Ubiquitin-dependent internalization and lysosomal degradation process.  NEDD4 also plays a role in facilitating the sequestration of alpha-synuclein to cellular endosomes.  This recombinant protein contains an N-terminal His6 tag and amino acids 570-1319 (UniProt P46934).

References
  1. Harvey K.F. & Kumar S (1999)  Trends Cell Biol.  9: 166
  2. Maspero E., et al. (2013)  Nat. Struct. Mol. Biol.  20: 696
  3. Scheffner M. & Kumar S. (2014)  Biochim. Biophys. Acta.  1843: 61
  4. Sugeno N., et al. (2014)  J. Biol. Chem.  289: 18137
  5. Yang B. & Kumar S (2010)  Cell Death Differ.  17: 68
Long Name
Neural Precursor Cell Expressed, Developmentally Down-regulated Protein 4
Entrez Gene IDs
4734 (Human); 17999 (Mouse); 25489 (Rat)
Alternate Names
Cell proliferation-inducing gene 53 protein; E3 ubiquitin-protein ligase NEDD4; EC 6.3.2; EC 6.3.2.-; KIAA0093NEDD4-1MGC176705; NEDD4; NEDD-4; Neural precursor cell expressed developmentally down-regulated protein 4; neural precursor cell expressed, developmentally down-regulated 4; receptor-potentiating factor 1; RPF1

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