Otubain-2 is a C65 type peptidase and a member of the ovarian tumor (OTU) protein super-family found in eukaryotes, viruses and pathogenic bacterium. Otubain-2 associates with the E3 ubiquitin ligases TRAF3 and TRAF6, and has been shown to negatively regulate virus-induced type I IFN induction and the antiviral responses mediated by these ligases. This recombinant protein contains a C-terminal 6-His tag.
Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF
R&D Systems | Catalog # E-554
Discontinued Product
E-554 has been discontinued.
View all Otubain-2 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Enzyme Activity
Loading...
Product Specifications
Source
E. coli-derived human Otubain-2 protein
Contains a C-terminal 6-His tag
Contains a C-terminal 6-His tag
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
28 kDa
Activity
Recombinant Human His6-Otubain-2 Isoform 1 is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-Otubain-2 Isoform 1 concentration of 0.1-2 μM.
Formulation, Preparation, and Storage
E-554
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 5 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Otubain-2
References
-
Li S., et al. (2010) J.Biol.Chem. 285: 4291-4297
- Nanao M.H., et al. (2004) EMBO Reports 5: 783-788
Long Name
OTU Domain, Ubiquitin Aldehyde Binding 2
Alternate Names
C14orf137, OTU2, OTUB2, Otubain2
Gene Symbol
OTUB2
UniProt
Additional Otubain-2 Products
Product Documents for Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF
For research use only
Related Research Areas
Customer Reviews for Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF and earn rewards!
Have you used Recombinant Human His6-Otubain-2 Isoform 1 Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Loading...
Associated Pathways
