Recombinant Human His6-OTUD7B/Cezanne Protein, CF

R&D Systems | Catalog # E-562

R&D Systems
Discontinued Product
E-562 has been discontinued. View all OTUD7B/Cezanne products.

Key Product Details

Source

Sf 21 (baculovirus)

Accession Number

Q6GQQ9

Applications

Bioactivity
View all OTUD7B/Cezanne Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human OTUD7B/Cezanne protein
Contains a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

93 kDa

Activity

Recombinant Human OTUD7B/Cezanne is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human OTUD7B/Cezanne concentration of 0.1-1 μM.

Formulation, Preparation, and Storage

E-562
Formulation

X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: OTUD7B/Cezanne

OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappaB (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa (1). The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked poly-Ubiquitin chains, and also to display a preference for K11-linked chains (1-3). It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3 (2,4).

References

  1. Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180.
  2. Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036.
  3. Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939.
  4. Hu, H. et al. (2013) Nature 494:371.

Long Name

OTU Domain-containing Protein 7B

Alternate Names

Cezanne, ZA20D1

Entrez Gene IDs

56957 (Human); 229603 (Mouse); 310677 (Rat)

Gene Symbol

OTUD7B

UniProt

Q6GQQ9

Additional OTUD7B/Cezanne Products

Product Documents for Recombinant Human His6-OTUD7B/Cezanne Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human His6-OTUD7B/Cezanne Protein, CF

For research use only

Citations for Recombinant Human His6-OTUD7B/Cezanne Protein, CF

Customer Reviews for Recombinant Human His6-OTUD7B/Cezanne Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human His6-OTUD7B/Cezanne Protein, CF and earn rewards!

Have you used Recombinant Human His6-OTUD7B/Cezanne Protein, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail