Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF

Catalog # Availability Size / Price Qty
1334-B7-025
Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF Data
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Product Details
Citations (5)
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Reviews (1)

Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF Summary

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind 6Ckine/CCL21 in a functional ELISA. Nagakubo, D. et al. (2003) J. Immunol. 171:553. When Recombinant Human (rh) IGFBP-rp1/IGFBP-7 (K95R) is immobilized at 500 ng/mL (100 μL/well), the concentration of Recombinant Human CCL21/6Ckine (Catalog # 366-6C) that produces 50% optimal binding response is found to be approximately 4-20 ng/mL.
Source
Mouse myeloma cell line, NS0-derived human IGFBP-rp1/IGFBP-7 protein
Asp30-Leu282 (K95R), with an N-terminal 10-His tag
Accession #
N-terminal Sequence
Analysis
His
Predicted Molecular Mass
27.5 kDa
SDS-PAGE
36 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1334-B7

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity View Larger

Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) (Catalog # 1334-B7) binds Recombinant CCL21/6Ckine in a functional ELISA. When Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) is immobilized at 500 ng/mL (100 µL/well), the concentration of Recombinant Human CCL21/6Ckine (Catalog # 366-6C) that produces 50% of the optimal binding response is approximately 4-20 ng/mL.

SDS-PAGE View Larger

1 μg/lane of Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 36 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IGFBP-rp1/IGFBP-7

IGFBP-rp1, also known as Mac25/Angiomodulin (AGM), tumor-derived adhesion factor (TAF) and prostacyclin-stimulating factor (PSF), is a secreted protein that contains three protein domain modules. Human IGFBP-rp1 cDNA encodes 282 amino acid (aa) residue precursor protein with a putative 26 aa signal peptide. Mature IGFBP-rp1 is a glycosylated protein with an N-terminal IGFBP domain, followed by a Kazal-type serine proteinase inhibitor domain and a C-terminal immunoglobulin-like C2-type domain. The similarity of IGFBP-rp1 with the IGFBPs is confined to the N-terminal IGFBP domain, which contains all 12 of the conserved cysteine residues found in IGFBP1 through 5. Human and mouse IGFBP-rP1 are highly homologous. Human and mouse IGFBP-rp1 share 94% aa sequence identity. IGFBP‑rp1 is expressed in many normal tissues and in cancer cells. It is abundantly expressed in high endothelial venules (HEVs) of blood vessels in the secondary lymphoid tissues. The expression of IGFBP-rp1 is upregulated in senescing epithelial cells and by retinoic acid. IGFBP-rp1 binds IGF and insulin with very low affinity and has been shown to enhance the mitogenic actions of IGF and insulin. IGFBP-rp1 also has IGF/insulin-independent activities. It interacts with heparan sulfate proteoglycans, type IV collagen, and specific chemokines. IGFBP-rp1 supports weak cell adhesion, promotes cell spreading on type IV collagen, and stimulates the production of the potent vasodilator PGI2.. It modulates tumor cell growth and has also been implicated in angiogenesis. IGFBP-rp1 is proteolytically cleaved between lysine 97 and alanine 98. Cleaved IGFBP-rp1 has enhanced cell attachment activity but can no longer bind IGF/insulin (1 - 3).

References
  1. Hwa, V. et al. (1999) Endocrinology Rev. 20:761.
  2. Nagakubo, D. et al. (2003) J. Immunol. 171:553.
  3. Ahmed, S. et al. (2003) Biochem. Biophys. Res. Commun. 310:612.
Long Name
Insulin-like Growth Factor Binding Protein Related Protein 1
Entrez Gene IDs
3490 (Human); 29817 (Mouse)
Alternate Names
angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor

Citations for Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Secretome analysis of in vitro aged human mesenchymal stem cells reveals IGFBP7 as a putative factor for promoting osteogenesis
    Authors: A Infante, CI Rodríguez
    Sci Rep, 2018;8(1):4632.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. IGFBP7 participates in the reciprocal interaction between acute lymphoblastic leukemia and BM stromal cells and in leukemia resistance to asparaginase.
    Authors: Laranjeira AB, de Vasconcellos JF, Sodek L, Spago MC, Fornazim MC, Tone LG, Brandalise SR, Nowill AE, Yunes JA
    Leukemia, 2011;26(5):1001-11.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.
    Authors: Gunawardana CG, Kuk C, Smith CR
    J. Proteome Res., 2009;8(10):4705-13.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  4. Insulin-like growth factor binding protein 7 mediates glioma cell growth and migration.
    Authors: Jiang W, Xiang C, Cazacu S, Brodie C, Mikkelsen T
    Neoplasia, 2008;10(12):1335-42.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Mannan-binding lectin-associated serine protease 3 cleaves synthetic peptides and insulin-like growth factor-binding protein 5.
    Authors: Cortesio CL, Jiang W
    Arch. Biochem. Biophys., 2006;449(1):164-70.
    Species: Human
    Sample Types: N/A
    Applications: Enzyme Assay

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Reviews for Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF

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Recombinant Human IGFBP-rp1/IGFBP-7 (K95R) Protein, CF
By Anonymous on 04/10/2019
Application: In vitro bioactivity in cell culture
Reason for Rating: We have use this to confirm previously published data that IGFBP-7 reduces matrigel tube formation.