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Recombinant Human IgG1 Fc Protein, CF

R&D Systems | Catalog # 110-HG

Analyzed by SEC-MALS
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Key Product Details

  • R&D Systems NS0-derived Recombinant Human IgG1 Fc Protein (110-HG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

P01857.1

Structure / Form

Dimer

Applications

Bioactivity
View all IgG1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human IgG1 protein
IEGRMD Human IgG1
(Pro100-Lys330)
Substitutions: Leu117Ala, Leu118Glu, Gly120Ala, Thr276Ala
Accession # P01857.1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ile

Predicted Molecular Mass

26.6 kDa

SDS-PAGE

30-35 kDa, reducing conditions

Activity

Bioassay data are not available.

Reviewed Applications

Read 10 reviews rated 4.7 using 110-HG in the following applications:

Scientific Data Images for Recombinant Human IgG1 Fc Protein, CF

Recombinant Human IgG1 Fc Protein SEC-MALS.

Recombinant Human IgG1/Fc Protein (Catalog # 110-HG) has a molecular weight (MW) of 55.8 kDa as analyzed by SEC-MALS, suggesting that this protein is a dimer. MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

Formulation, Preparation, and Storage

110-HG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

It is recommended that sterile PBS containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 100 μg/mL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG1

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G1

Alternate Names

IgG1, Igh-4, RGD1359539, VH7183

UniProt

P01857.1

Additional IgG1 Products

Product Documents for Recombinant Human IgG1 Fc Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human IgG1 Fc Protein, CF

For research use only

Citations for Recombinant Human IgG1 Fc Protein, CF

Customer Reviews for Recombinant Human IgG1 Fc Protein, CF (10)

4.7 out of 5
10 Customer Ratings
5 Stars
70%
4 Stars
30%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 of 10 reviews Showing All
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  • Use of Fc Fragment in a Competition Assay to Assess Binding in MSD
    Name: Anonymous
    Application: Elisa/MSD
    Species: Human
    Verified Customer | Posted 04/06/2026
    The assay signal is Fc-dependent, as demonstrated by competition with the Fc fragment.
    The Fc fragment was evaluated in a Protein G-based MSD competition assay to confirm assay specificity. When added to the assay, the Fc competed with the sample IgG for Protein G binding, resulting in a dose-dependent reduction of signal.
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Recombinant Human IgG1 Fc Protein, CF
    Name: Anonymous
    Application: size exclusion chromatography
    Verified Customer | Posted 12/12/2024
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Recombinant Human IgG1 Fc Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 10/06/2021
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Recombinant Human IgG1 Fc Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 10/26/2020
    I am using it as my negative control in the cell adhesion assay.
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Recombinant Human IgG1 Fc Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/04/2020
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Name: tenny tang
    Application: Cell Proliferation
    Verified Customer | Posted 12/21/2018
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Name: tenny tang
    Application: Cell Proliferation
    Verified Customer | Posted 12/07/2018
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Name: tenny tang
    Application: Cell Adhesion
    Verified Customer | Posted 10/13/2018
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Recombinant Human IgG1 Fc, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/08/2017
    Recombinant Human IgG1 Fc Protein, CF 110-HG
  • Name: Anonymous
    Verified Customer | Posted 04/21/2017

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FAQs for Recombinant Human IgG1 Fc Protein, CF

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  • Q: Recombinant Human IgG1 Fc Protein, CF (Catalog # 110-HG) has 4 mutations: Leu117Ala, Leu118Glu, Gly120Ala, and Thr276Ala. What is the purpose of these mutations?

    A: The mutations in the 110-HG protein sequence were introduced to aid in the stability of the protein.

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