Recombinant Human IGSF21 His-tag Protein, CF

Catalog # Availability Size / Price Qty
Recombinant Human IGSF21 His-tag Protein Binding Activity
2 Images
Product Details

Recombinant Human IGSF21 His-tag Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), it binds to Recombinant Human Neurexin 2 alpha Fc Chimera (Catalog # 6636-NX) with an ED50 of 20-120 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human IGSF21 protein
Tyr25-Thr455, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass

49 kDa

61-68 kDa, under reducing conditions

Product Datasheets

You must select a language.



Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Human IGSF21 His-tag Protein Binding Activity View Larger

When Recombinant IGSF21 His-tag (Catalog # 10330-S2) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human Neurexin 2 alpha Isoform 2 Fc Chimera (Catalog # 6636-NX) binds with an ED50 of 20-120 ng/mL.

SDS-PAGE Recombinant Human IGSF21 His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Human IGSF21 His-tag (Catalog # 10330-S2) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 61-68 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: IGSF21

IGSF21 (Immunoglobulin superfamily member 21) is an approximately 60 kDa glycoprotein that is a member of the immunoglobulin superfamily. In mouse, two isoforms have been identified. The long isoform is predominantly expressed in the brain, while the short isoform ~35 kDa is expressed in other organs (1). IGSF21 is expressed at both embryonic and postnatal stages (1). Human IGSF21 is synthesized as a 467 amino acid (aa) protein that includes a 24 aa signal peptide. The mature protein, which contains two Ig-like domains, shares 95% aa sequence identity with mouse and rat IGSF21. IGSF21 selectively promotes inhibitory presynaptic differentiation through interaction with presynaptic neurexin2 alpha that may represent an important mechanism of inhibitory synapse development in the brain (1). Thalamic IGSF21 expression is altered in response to FGF8 signaling (2). Genotypes of IGSF21 are also reported to influence the development of diabetic retinopathy (3).

  1. Tanabe, Y. et al. (2017) Nat. Commun. 8:408.
  2. Botella-Lopez, A. et al. (2019) Brain Struct. Funct. 224:661.
  3. Lin, X. et al. (2016) J. Gene Med. 18:282.
Long Name
Immunoglobulin Superfamily, Member 21
Entrez Gene IDs
84966 (Human); 230868 (Mouse); 298591 (Rat)
Alternate Names
FLJ41177; IGSF21; immunoglobin superfamily, member 21; immunoglobulin superfamily member 21; MGC15730; RP11-121A23.1


No product specific FAQs exist for this product, however you may

View all Proteins and Enzyme FAQs

Reviews for Recombinant Human IGSF21 His-tag Protein, CF

There are currently no reviews for this product. Be the first to review Recombinant Human IGSF21 His-tag Protein, CF and earn rewards!

Have you used Recombinant Human IGSF21 His-tag Protein, CF?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review