Recombinant Human IGSF4C/SynCAM4 His tagged Protein, CF

Catalog # Availability Size / Price Qty
4164-S4-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human IGSF4C/SynCAM4 His tagged Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Optimal neurite outgrowth was observed when neurons were plated on nitrocellulose coated 96-well culture plates that had been pre-coated with 50 μL/well of Recombinant Human IGSF4C/SynCAM4 at 6-24 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human IGSF4C/SynCAM4 protein
Gln25-Tyr323, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln25 predicted
Predicted Molecular Mass
34 kDa
SDS-PAGE
50-65 kDa, reducing conditions

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4164-S4

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4164-S4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IGSF4C/SynCAM4

IGSF4C is an immunoglobulin superfamily member that is also known as Nectin-like protein 4 (Necl-4), synaptic cell adhesion molecule 4 (SynCAM4), or tumor suppressor in lung cancer-like 2 (TSLL-2) (1, 2). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in neurons, but also in lung, kidney, bladder, prostate and testis (1-5). Their extracellular domains (ECD) share 35-50% amino acid (aa) identity and each contain one V-type Ig-like and two C2-type Ig-like domains. These domains are responsible for Ca++-independent homophilic and heterophilic interactions. The 388 aa human IGSF4C contains a 20 aa signal sequence, a 304 aa ECD that shares 98-99% amino acid identity with mouse, rat, canine and bovine IGSF4C, a 21 aa transmembrane sequence, and a 43 aa cytoplasmic domain. The apparent size of mouse or human IGSF4C may be variably reported as 48-67 kDa, probably due to differences in glycosylation (2, 5, 8). In the peripheral nervous system, IGSF4C is expressed on Schwann cells, and its internodal interaction with IGSF4A (Necl-1, SynCAM-3) on axons is critical for adhesion and myelination (6-8). In the brain, all IGSF4 family members are expressed at high levels concurrent with synapse formation (4). In the cerebellum, IGSF4C is expressed on Purkinje cells, with complementary expression of IGSF4 on granule cells (4). Heterophilic interaction with IGSF4D (Necl-3, SynCAM2) has also been identified, but homophilic interaction is unlikely (4, 6). IGSF4C is also proposed as a tumor suppressor that is downregulated in many prostate cancers and gliomas (1, 5).

References
  1. Fukuhara, H. et al. (2001) Oncogene 20:5401.
  2. Biederer, T. et al. (2006) Genomics 87:139.
  3. Takai, Y. et al. (2008) Nat. Rev. Mol. Cell Biol. 9:603.
  4. Thomas, L. A. et al. (2008) J. Comp. Neurol. 510:47.
  5. Williams, Y. N. et al. (2005) Oncogene 25:1446.
  6. Fogel, A. I. et al. (2007) J. Neurosci. 27:12516.
  7. Spiegel, I. et al. (2007) Nat. Neurosci. 10:861.
  8. Maurel, P. et al. (2007) J. Cell Biol. 178:861.
Long Name
Immunoglobulin Superfamily, Member 4B
Entrez Gene IDs
199731 (Human); 260299 (Mouse)
Alternate Names
CADM4; cell adhesion molecule 4; IGSF4C; IGSF4Cnectin-like 4; Immunoglobulin superfamily member 4C; immunoglobulin superfamily, member 4C; Necl-4; NECL4synCAM4; Nectin-like protein 4; SynCAM4; TSLC1-like 2; TSLC1-like protein 2; TSLL2; TSLL2SynCAM4

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