Recombinant Human IGSF4D/CADM2 Protein, CF

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R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human IGSF4D/CADM2 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. Able to significantly enhance neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.
Mouse myeloma cell line, NS0-derived human IGSF4D/CADM2 protein
Accession # AAI06000
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Predicted Molecular Mass
35.5 kDa (monomer)
36-55 kDa, reducing conditions

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Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IGSF4D/CADM2

IGSF4D is an immunoglobulin superfamily member that is also designated as synaptic cell adhesion molecule 2 (SynCAM 2) and nectin-like protein 3 (Necl-3) (1). The four IGSF4 proteins, designated A, B, C and D, are type I transmembrane glycoproteins expressed mainly in epithelial cells that contain one V-type Ig-like and two C2-type Ig-like domains in their extracellular regions. These domains are characteristically responsible for Ca++-independent homophilic and heterophilic interactions in the brain, lung, kidney, bladder, prostate and testis (1-3). Based on homology with other family members, the 437 amino acid (aa) human IGSF4D contains a 355 aa extracellular domain (ECD) that shares > 99% aa identity with corresponding regions of canine and bovine, and 93% aa identity with rat and mouse IGSF4D. Rat and mouse IGSF4D lack an exon at the end of the putative ECD. If this exon is disregarded, aa identity with human IGSF4D rises to > 98%. IGSF4A, B, C and D proteins share 35‑50% aa identity within the ECD. Amino acids found to be critical in other IGSF4 proteins for adhesion and intracellular binding of MAGUK guanylate kinase subfamily proteins are conserved in IGSF4D (3). The expression and function of IGSF4D has not been well characterized. However, in-house quality control data at R&D Systems has shown that, like other family members, IGSF4D has a positive effect on in vitro outgrowth of cortical neurons.

  1. Biederer, T. et al. (2006) Genomics 87:139.
  2. Sakisaka T. and Y. Takai (2004) Curr. Opin. Cell Biol. 16:513.
  3. Kakunaga, S. et al. (2004) J. Cell Sci. 118:1267.
Long Name
Immunoglobulin Superfamily, Member 4D
Entrez Gene IDs
253559 (Human); 239857 (Mouse)
Alternate Names
CADM2; cell adhesion molecule 2; IGSF4D; IGSF4DMGC138343; Immunoglobulin superfamily member 4D; immunoglobulin superfamily, member 4D; MGC104534; Necl-3; NECL3MGC138341; nectin-like 3; Nectin-like protein 3; SynCAM2


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