Recombinant Human KAL1 Protein, CF

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Recombinant Human KAL1 Protein Bioactivity
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Recombinant Human KAL1 Protein, CF Summary

Product Specifications

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Measured by its binding ability in a functional ELISA. When Recombinant Human FGF basic 146 aa Recombinant Human FGF basic 146 aa (Catalog # 233-FB) is coated at 1.5 µg/mL, 100 μL/well, Recombinant Human KAL1 binds with an ED50 of 8-48 ng/mL.
Chinese Hamster Ovary cell line, CHO-derived human KAL1 protein
Ala25-Tyr680, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Predicted Molecular Mass
75 kDa
84-103 kDa, reducing conditions

Product Datasheets

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Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.


Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose.
Reconstitution Reconstitute at 200 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Human KAL1 Protein Bioactivity View Larger

When Recombinant Human FGF basic 146 aa (Catalog # 233-FB) is coated at 1.5 µg/mL, Recombinant Human KAL1 (Catalog # 9314-KA) binds with an ED50 of 8-48 ng/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Background: KAL1

Anosmin-1, also known as Kal-1, is a 100 kDa secreted glycoprotein that regulates neuronal adhesion and migration. Mutations in Anosmin-1 are associated with the development of X-linked Kallman syndrome which can be accompanied by hearing loss (1). Mature human Anosmin-1 contains one WAP domain and four Fibronectin type-III domains (2). It is expressed by cerebellar Purkinje cells and deep nuclear neurons (2, 3), piriform cortex and olfactory bulb mitral cells (2, 4), optic tectum and retinal ganglion axons (2, 5, 6), and oligodendrocyte progenitor cells (OPC) (4). Anosmin-1 binds to Integrin beta 1/CD29, FGF R1, L1CAM, and complexes of FGF R1 with FGF basic and heparan sulfate proteoglycans, and it enhances signaling through FGF R1 (6-9). It mediates OPC adhesion, inhibits FGF basic-induced OPC migration, and enhances oligodendrocyte-mediated axon myelination (5, 6, 10, 11). It also cooperates with FGF basic to promote olfactory axon elongation and neurite outgrowth (3, 4, 8, 9, 12, 13). In addition, Anosmin-1 is up-regulated in glioblastoma and gastric cancer where it enhances tumor cell proliferation and migration (7, 14).

  1. Korsensky, L. and D. Ron (2016) Semin. Cell Dev. Biol. 53:101.
  2. Soussi-Yanicostas, N. et al. (1996) J. Cell Sci. 109:1749.
  3. Gianola, S. et al. (2009) Neuroscience 158:570.
  4. Soussi-Yanicostas, N. et al. (2002) Cell 109:217.
  5. Bribian, A. et al. (2008) Dev. Neurobiol. 68:1503.
  6. Bribian, A. et al. (2006) Mol. Cell. Neurosci. 33:2.
  7. Choy, C.T. et al. (2013) Endocr. Relat. Cancer 21:85.
  8. Diaz-Balzac, C.A. et al. (2015) Cell Rep. 11:1377.
  9. Gonzalez-Martinez, D. et al. (2004) J. Neurosci. 24:10384.
  10. Clemente, D. et al. (2011) J. Neurosci. 31:14899.
  11. Murcia-Belmonte, V. et al. (2016) Brain Struct. Funct. 221:1365.
  12. Soussi-Yanicostas, N. et al. (1998) J. Cell Sci. 111:2953.
  13. Garcia-Gonzalez, D. et al. (2016) Brain Struct. Funct. 221:239.
  14. Kanda, M. et al. (2016) Int. J. Cancer 138:721.
Alternate Names
CDK5 regulatory subunit associated protein 1-like 1; CDK5 regulatory subunit-associated protein 1-like 1; FLJ20342; FLJ46705; MGC75469


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