Recombinant Human LAMP Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
873-LP-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human LAMP Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic hippocampal neurons. Able to significantly enhance neurite outgrowth when immobilized at 10 µg/mL.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human LAMP protein
Human LAMP
(Val29 - Asn315)
Accession # AAC50569
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val29
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
58.4 kDa (monomer)
SDS-PAGE
70-75 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

873-LP

Formulation Supplied as a 0.2 μm filtered solution in PBS.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
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Background: LAMP

LAMP (limbic system-associated membrane protein) is a member of the IgLON (immunoglobulin LAMP, OBCAM and neurotrimin) subfamily within the Ig superfamily. All IgLON family members are glycosylphosphatidylinositol (GPI)-anchored neural cell adhesion molecules that are involved in cell-cell recognition and may have a role in mediating selective neuronal growth and axon targeting. LAMP cDNA encodes a 338 amino acid (aa) residues precursor protein containing a 28 aa N-terminal signal peptide, a 23 aa C-terminal propeptide and a 287 aa mature chain with 3 Ig-like C2-type domains and a GPI-anchor attachment site. In the developing brain, LAMP exhibits a specific pattern of expression in the cortical and subcortical limbic areas, which are important in cognition, emotion, memory, and learning. LAMP is also expressed in single layers of the superior colliculus, spinal chord and cerebellum. LAMP promotes adhesion and growth of limbic axons primarily via homophilic interaction and in part by modulating calcium influx through L-type calcium channels in limbic neurons. Heterophilic interactions between LAMP and neurotrimin have also been demonstrated. LAMP has been shown to inhibit the outgrowth of neurotrimin-expressing dorsal root ganglion neurons in a heterophilic manner. Antibody perturbation studies showed that LAMP is necessary for normal circuit formation in the limbic system, including the septo-hippocampal connection and hippocampal mossy fibers. LAMP acts as an attractive guidance signal for the limbic thalamic axons and can induce branch formation, but also acts as a repulsive axon guidance signal for nonlimbic thalamic axons (1 - 5).

References
  1. Pimenta, A.F. et al. (1995) Neuron, 15:287.
  2. Zhukareva, V. and P. Levitt (1995) Development 121:1161.
  3. Zhukareva, V. et al. (1997) Mol. Cell Neurosci. 10:43.
  4. Mann, F. et al. (1998) Journal Neurosci. 18:9409.
  5. Gil, O.D. et al. (2002) J. Neurobiol. 51:190.
Long Name
Limbic System-associated Membrane Protein
Entrez Gene IDs
4045 (Human)
Alternate Names
FLJ34254; FLJ35396; IgLON family member 3; IGLON3FLJ54658; LAMP; LAMPFLJ37216; limbic system-associated membrane protein; LSAMP

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