Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF Summary
Arg35-Leu289, with a C-terminal 6-His tag
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
When Recombinant Human Osteopontin (OPN) Protein (Catalog # 1433-OP/CF) is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin (aa 35-289) His-tag Protein (Catalog # 10093-MS) that produces 50% of the optimal binding response is 0.003-0.018 µg/mL.
2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 27-39 kDa.
Megakaryocyte-Potentiating Factor (MPF), is derived from a 70 kDa Mesothelin precursor (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic furin cleavage site between aa 288-293. The cleavage releases soluble N-terminal MPF (aa 37-286) and C-terminal Mesothelin (aa 296-606) remains attached to the cell surface via a GPI linkage. (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). This recombinant human MPF shares 56% sequence identity with mouse and rat MPF. Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).
- Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
- Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
- Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
- Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
- Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
- Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.
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