Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF

N-Terminal
Catalog # Availability Size / Price Qty
10093-MS-050
Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF Binding Activity
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Recombinant Human Mesothelin (aa 35-289) His-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human CA125/MUC16 (Catalog # 5609-MU) is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog # 10093-MS) that produces 50% of the optimal binding response is 0.35-2.1 μg/mL.
Source
Mouse myeloma cell line, NS0-derived human Mesothelin protein
Arg35-Leu289, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Arg35
Structure / Form
Monomer
Predicted Molecular Mass
28 kDa
SDS-PAGE
27-39 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10093-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Binding Activity Binding Activity View Larger

When Recombinant Human CA125/MUC16 (Catalog # 5609-MU) is immobilized at 4 µg/mL, 100 µL/well, Recombinant Human Mesothelin N-Terminal(aa 35-289) His-tag (Catalog # 10093-MS) binds with an ED50 of 0.35-2.1 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Human Mesothelin N-Terminal (aa 35-289) His-tag (Catalog# 10093-MS) was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 27-39 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Mesothelin

Megakaryocyte-Potentiating Factor (MPF), is derived from a 70 kDa Mesothelin precursor (1-3). The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic furin cleavage site between aa 288-293. The cleavage releases soluble N-terminal MPF (aa 37-286) and C-terminal Mesothelin (aa 296-606) remains attached to the cell surface via a GPI linkage. (3, 4). MPF is a cytokine that potentiates IL-3 induced megakaryocyte colony formation (2, 5). This recombinant human MPF shares 56% sequence identity with mouse and rat MPF. Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).

References
  1. Hassan, R. et al. (2004) Clin. Cancer Res. 10:3937.
  2. Kojima, T. et al. (1995) J. Biol. Chem. 270:21984.
  3. Chang, K. and I. Pastan (1996) Proc. Natl. Acad. Sci. 93:136.
  4. Onda, M. et al. (2006) Clin. Cancer Res. 12:4225.
  5. Yamaguchi, N. et al. (1994) J. Biol. Chem. 269:805.
  6. Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.
Entrez Gene IDs
10232 (Human); 56047 (Mouse); 60333 (Rat)
Alternate Names
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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