Recombinant Human MUC-1 Fc Chimera Alexa Fluor® 647 Protein

Catalog # Availability Size / Price Qty
AFR10332-020

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Flow cytometry analysis for Recombinant Human MUC-1 Fc Chimera Alexa Fluor® 647 staining on anti-Human MUC-1 Monoclonal Antibody conjugated beads
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Recombinant Human MUC-1 Fc Chimera Alexa Fluor® 647 Protein Summary

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Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by flow cytometry for its ability to bind anti-Human MUC-1 Monoclonal Antibody conjugated beads.The concentration of Recombinant human MUC-1 Chimera Alexa Fluor® 647 (Catalog# AFR10332) that produces 50% of the binding response is 0.5-10 ng/mL
Source
Human embryonic kidney cell, HEK293-derived human MUC-1 protein
Human MUC-1
(Ser24-Ser380)
Accession # P15941.3
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Ser24
Structure / Form
Labeled with Alexa Fluor® 647

Excitation Wavelength: 650 nm  
Emission Wavelength: 668 nm
Predicted Molecular Mass
60 kDa
SDS-PAGE
157-192 kDa, under reducing conditions

Product Datasheets

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AFR10332

AFR10332

Formulation Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Scientific Data

Flow Cytometry View Larger

Streptavidin coated beads conjugated to biotinylated anti-Human MUC-1 Monoclonal Antibody were stained with the indicated concentrations of Recombinant Human MUC-1 Fc Chimera Alexa Fluor® 647 (Catalog # AFR10332).

SDS-PAGE View Larger

2 μg/lane of Recombinant Human MUC-1 Fc Chimera Alexa Fluor® 647 Protein (Catalog # AFR10332) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 157-192 kDa and 310-380 kDa, respectively.

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Background: MUC-1

MUC-1 (Mucin-1) is a type 1 transmembrane glycoprotein that is normally expressed on the apical surface of most epithelial cells (1-2). It is known to be overexpressed by various human carcinomas and is shed into circulation (2). The extracellular domain is made up of tandem repeats (TRs) of 20 aa each, with each TR containing five potential O-glycosylation sites (3). The number of TRs vary between 25-100, depending on the allele (3). Within the mature region including 16 TRs (residues 24-380), human MUC-1 shares 30% aa sequence identity with mouse and rat MUC-1. It has been reported that high expression level of MUC-1 generally correlates with increased mortality rates (4). In addition, MUC-1 is aberrantly underglycosylated on cancer cells with short and sialylated O-linked glycans in contrast to the long, branched chain seen in normal epithelial cells (4-7). It has been demonstrated that MUC-1 can interact with E-selectin and ICAM-1 to mediate firm adhesion of circulating tumor cells and subsequent extravasation in the metastatic adhesion cascade (4). Furthermore, MUC-1 can modulate the tumor immunological microenvironment through engagement of Siglec-9 by inducing the recruitment of beta-catenin to the cytoplasmic tail of MUC-1, increasing the expression of PD-L1 by macrophages, and activating the MEK-ERK pathway (5,6). MUC-1 can also interact with Galectin-3 to promote EGFR activation thus regulating EGFR-associated tumorigenesis and cancer progression (7).

References
  1. Rughetti, A. et al. (2005) J. Immunol. 174:7764.
  2. Engelstaedter, V. et al. (2012) BMC Cancer 12:600.
  3. Taylor-Papadimitriou, J. et al. (1999) Biochim. Biophys. Acta 1455:301.
  4. Geng, Y. et al. (2012) Front Oncol. 2:76.
  5. Tanida, S. et al. (2013) J Biol Chem. 288:31842.
  6. Beatson, R. et al. (2016) Nat Immunol. 17:1273.
  7. Piyush, T. et al. (2017) Cell Death Differ. 24:1937.
Long Name
Mucin 1, Cell Surface-associated
Entrez Gene IDs
4582 (Human)
Alternate Names
Breast carcinoma-associated antigen DF3; Carcinoma-associated mucin; CD227 antigen; CD227; DF3 antigen; EMA; Episialin; H23 antigen; H23AG; KL-6; MAM6; MUC-1; MUC1/ZD; mucin 1, cell surface associated; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMMUC-1/SEC; PEMT; Polymorphic epithelial mucin; PUMMUC-1/X; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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