Recombinant Human NETO1 His-tag Protein, CF Summary
Thr23-Val345, with a C-terminal 6-His tag
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CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
2 μg/lane of Recombinant Human NETO1 His-tag Protein (Catalog # 11103-NO) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 34-48 kDa.
Neuropilin and tolloid-like protein 1 (NETO1), also known as Brain-specific transmembrane protein containing 2 CUB and 1 LDL-receptor class A domains protein 1 (BTCL1), is a type I transmembrane protein that is expressed in the brain and retina (1). The 511 amino acid (aa) residue mature human NETO1 contains a 322 aa extracellular domain (ECD) with two CUB domains and one class A LDL-receptor segment, a 21 aa transmembrane domain, and a 168 aa cytosol domain (1). Three human NETO1 splice isoforms exist, including a secreted soluble variant that shows an Asp-to-Glu transition at residue 157 followed by a premature truncation (1). The ECD of human NETO1 shares 97% and 98% aa identity with that of mouse and canine NETO1, respectively. NETO1 is expressed in the human brain and retina (1, 2). It increases the rate of GluK1 receptor desensitization and is a candidate NMDAR auxiliary subunit regulating channel activities and synaptic transmission (3, 4). NETO1 expression is associated with metastasis and poor survival in epithelial ovarian cancer (5).
- Stöhr, H. et al. (2002) Gene 286:223.
- Tomita, S. and P.E. Castillo (2012) J. Physiol. 590:2217.
- Copits, B.A. et al. (2011) J. Neurosci. 31:7334.
- Ng, D. et al. (2009) PloS Biol. 7:e41.
- Xu, Y. et al. (2020) J. Cell. Mol. Med. 24:9114.
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