Recombinant Human Neuroligin 2/NLGN2 Protein, CF

Catalog # Availability Size / Price Qty
5645-NL-050

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Recombinant Human Neuroligin 2/NLGN2 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Neuroligin 2/NLGN2 is immobilized at 1.5 μg/mL, Recombinant Human Neurexin 1 beta /NXRN1b Fc Chimera (Catalog # 5268-NX) binds with an apparent K<10 nM.
Source
Mouse myeloma cell line, NS0-derived human Neuroligin 2/NLGN2 protein
Gln15-Ser660, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln15 predicted
Predicted Molecular Mass
72 kDa
SDS-PAGE
85-95 kDa, reducing conditions

Product Datasheets

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5645-NL

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

5645-NL

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 400 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: Neuroligin 2/NLGN2

Neuroligin 2 (NLGN2) is one of several type I transmembrane Neuroligins that are expressed on neuronal postsynaptic densities. Neuroligins play an important role in synaptic development and function (1). Mature human Neuroligin 2 is a 105 kDa protein that consists of a 663 amino acid (aa) extracellular domain (ECD) with a catalytically inactive cholinesterase-like domain, a 21 aa transmembrane segment, and a 137 aa cytoplasmic tail (2, 3). Within the ECD, human Neuroligin 2 shares 98% aa sequence identity with mouse and rat Neuroligin 2. Alternate splicing generates an isoform with a 17 aa insertion at splice site A within the ECD (2). This recombinant protein does not contain the splice site A insert (-SS A). Neuroligin 2 is expressed on neurons in the brain and also on pancreatic beta  cells where it facilitates insulin secretion (2, 4, 5). The -SS A isoform is uniformly expressed among inhibitory and excitatory synapses, while the +SS A isoform is enriched at inhibitory GABAergic synapses (4, 6, 7). Neuroligin 2 clusters at postsynaptic densities in association with other postsynaptic proteins including S-SCAM, PSD-95, gephyrin, and Neuroligin 3 (4, 8-10). Synaptic maturation is promoted by the binding of Neuroligin 2 with presynaptic Neurexins, and these interactions are restricted to particular combinations of isoforms of the binding partners (11-15). Neuroligin 2 interacts with the alpha and beta forms of Neurexin 1, 2, and 3 (14). Its -SS A and +SS A isoforms are bound equally well by Neurexin 1 beta isoforms (-SS4 or +SS4), although only the Neurexin 1 beta +SS4 isoform can induce development of Neuroligin 2-dependent GABAergic contacts (7, 15).

References
  1. Sudhof, T.C. (2008) Nature 455:903.
  2. Ichtchenko, K. et al. (1996) J. Biol. Chem. 271:2676.
  3. Koehnke, J. et al. (2008) Proc. Natl. Acad. Sci. 105:1873.
  4. Varoqueaux, F. et al. (2004) Eur. J. Cell Biol. 83:449.
  5. Suckow, A.T. et al. (2008) Endocrinology 149:6006.
  6. Graf, E.R. et al. (2004) Cell 119:1013.
  7. Chih, B. et al. (2006) Neuron 51:171.
  8. Sumita, K. et al. (2007) J. Neurochem. 100:154.
  9. Irie, M. et al. (1997) Science 277:1511.
  10. Budreck, E.C. and P. Scheiffele (2008) Eur. J. Neurosci. 26:1738.
  11. Chih, B. et al. (2005) Science 307:1324.
  12. Chubykin, A.A. et al. (2007) Neuron 54:919.
  13. Hines, R.M. et al. (2008) J. Neurosci. 28:6055.
  14. Kang, Y. et al. (2008) J. Biol. Chem. 283:2323.
  15. Graf, E.R. et al. (2006) J. Neurosci. 26:4256.
Entrez Gene IDs
57555 (Human); 216856 (Mouse); 117096 (Rat)
Alternate Names
EC 3.1.1; EC 3.1.1.1; KIAA1366neuroligin-2; Neuroligin 2; NLGN2

Citations for Recombinant Human Neuroligin 2/NLGN2 Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Neuroligin-1 in brain and CSF of neurodegenerative disorders: investigation for synaptic biomarkers
    Authors: E Camporesi, T Lashley, J Gobom, J Lantero-Ro, O Hansson, H Zetterberg, K Blennow, B Becker
    Acta neuropathologica communications, 2021;9(1):19.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Reference Standard
  2. Interaction of A? Oligomers with Neurexin 2? and Neuroligin 1 Mediates Synapse Damage and Memory Loss in Mice
    Authors: J Brito-More, MV Lourenco, MM Oliveira, JF Vital, FC Ribeiro, JH Ledo, LP Diniz, MH Magdesian, HM Melo, F Barros-Ara, JM de Souza, SV Alves-Leon, FC Gomes, JR Clarke, CP Figueiredo, FG De Felice, ST Ferreira
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Types: Protein
    Applications: Bioassay

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