OTU domain-containing protein 3 (OTUD3) is a specialized cysteine protease and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 45 kDa. The human protein shares 83% amino acid sequence identity with its mouse ortholog. OTUD3 is exceedingly well characterized in terms of the structure-function relationship that results in the enzymes preferred polyubiquitin substrates. This untagged recombinant protein contains the catalytic (OTU) and UBA-like (Ubiquitin Associated) domains of OTUD3, and demonstrates a strong preference for K6- and K11-linked polyubiquitin chains.
Recombinant Human OTUD3 Catalytic Domain Protein, CF
R&D Systems | Catalog # E-574
Discontinued Product
E-574 has been discontinued.
View all OTUD3 products.
Key Product Details
Source
E. coli
Accession Number
Applications
Bioactivity
Loading...
Product Specifications
Source
E. coli-derived human OTUD3 protein
Glu52 - Gly275
Glu52 - Gly275
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
26 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human OTUD3 Catalytic Domain concentration of 0.05-0.5 μM.
Formulation, Preparation, and Storage
E-574
| Formulation | 1.3 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10 % Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: OTUD3
References
- Mevissen, T.E.T. et al. (2013) Cell 154: 169
Long Name
OTU Domain-containing Protein 3
Alternate Names
DUBA4, KIAA0459
Gene Symbol
OTUD3
UniProt
Additional OTUD3 Products
Product Documents for Recombinant Human OTUD3 Catalytic Domain Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human OTUD3 Catalytic Domain Protein, CF
For research use only
Related Research Areas
Citations for Recombinant Human OTUD3 Catalytic Domain Protein, CF
Powered by Bioz
Customer Reviews for Recombinant Human OTUD3 Catalytic Domain Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Human OTUD3 Catalytic Domain Protein, CF and earn rewards!
Have you used Recombinant Human OTUD3 Catalytic Domain Protein, CF?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Loading...
Associated Pathways
