Recombinant Human OTUD3 Catalytic Domain Protein, CF

Catalog # Availability Size / Price Qty
E-574-050
Product Details
Citations (1)
FAQs
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Recombinant Human OTUD3 Catalytic Domain Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human OTUD3 Catalytic Domain concentration of 0.05-0.5 μM.

Source
E. coli-derived human OTUD3 protein
Glu52 - Gly275
Accession #
Predicted Molecular Mass
26 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-574

Formulation

1.3 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10 % Glycerol (v/v), 1 mM TCEP

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OTUD3

OTU domain-containing protein 3 (OTUD3) is a specialized cysteine protease and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 45 kDa. The human protein shares 83% amino acid sequence identity with its mouse ortholog. OTUD3 is exceedingly well characterized in terms of the structure-function relationship that results in the enzymes preferred polyubiquitin substrates. This untagged recombinant protein contains the catalytic (OTU) and UBA-like (Ubiquitin Associated) domains of OTUD3, and demonstrates a strong preference for K6- and K11-linked polyubiquitin chains.

References
  1. Mevissen, T.E.T. et al. (2013) Cell 154: 169
Long Name
OTU Domain-containing Protein 3
Entrez Gene IDs
23252 (Human); 73162 (Mouse); 500572 (Rat)
Alternate Names
DUBA4; KIAA0459; OTU domain containing 3; OTUD3

Citation for Recombinant Human OTUD3 Catalytic Domain Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Activity-Based Probes for HECT E3 ubiquitin ligases
    Authors: R Byrne, T Mund, J Licchesi
    Chembiochem, 2017;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay

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