Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

R&D Systems | Catalog # E-563

R&D Systems
Discontinued Product
E-563 has been discontinued. View all OTUD7B/Cezanne products.

Key Product Details

Source

E. coli

Accession Number

Q6GQQ9

Applications

Bioactivity
View all OTUD7B/Cezanne Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human OTUD7B/Cezanne protein
Asn124 - Leu436

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

36 kDa

Activity

Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Cezanne Catalytic Domain concentration of 0.1-1.0 μM.

Formulation, Preparation, and Storage

E-563
Formulation

1.8 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10 % Glycerol (v/v), 1 mM TCEP
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: OTUD7B/Cezanne

OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappa B (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa. The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked polyubiquitin chains, but this enzyme displays a very strong preference for K11-linked chains in vitro. It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3. This untagged recombinant protein contains the catalytic domain (including the OTU domain) of Cezanne.

References

  1. Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939
  2. Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036
  3. Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180
  4. Hu, H. et al. (2013) Nature 494: 371

Long Name

OTU Domain-containing Protein 7B

Alternate Names

Cezanne, ZA20D1

Entrez Gene IDs

56957 (Human); 229603 (Mouse); 310677 (Rat)

Gene Symbol

OTUD7B

UniProt

Q6GQQ9

Additional OTUD7B/Cezanne Products

Product Documents for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

For research use only

Citations for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

Customer Reviews for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF

There are currently no reviews for this product. Be the first to review Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF and earn rewards!

Have you used Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Proteins and Enzymes

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail