Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

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137-PS-050
R&D Systems Recombinant Proteins and Enzymes
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Citations (45)
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Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. P-Selectin/Fc Chimera, immobilized at 10 µg/mL, will induce greater than 50% adhesion on U937 cells (100 µL/well at 1 x 106 cells/mL).
Optimal dilutions should be determined be each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived human P-Selectin/CD62P protein
Human P-Selectin
(Trp42 - Ala771)
Accession # P16109
IEGRDMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Trp42
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
106 kDa (monomer)
SDS-PAGE
146-160 kDa, reducing conditions

Product Datasheets

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137-PS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

137-PS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: P-Selectin/CD62P

Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Human P-Selectin cDNA encodes an 830 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 730 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH2-terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

References
  1. Kansas, G.S. (1996) Blood 88:3259.
  2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.
Entrez Gene IDs
6403 (Human); 20344 (Mouse)
Alternate Names
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Citations for Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

45 Citations: Showing 1 - 10
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  1. Oligomer-guided recognition of two fucan sulfate from Bohadschia argus and inhibition of P-selectin binding to its ligand
    Authors: Ruzemaimaiti, D;Sun, H;Zhang, J;Xu, C;Chen, L;Yin, R;Zhao, J;
    Carbohydrate polymers
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  2. An isoform of the giant protein titin is a master regulator of human T lymphocyte trafficking
    Authors: Toffali, L;D'Ulivo, B;Giagulli, C;Montresor, A;Zenaro, E;Delledonne, M;Rossato, M;Iadarola, B;Sbarbati, A;Bernardi, P;Angelini, G;Rossi, B;Lopez, N;Linke, WA;Unger, A;Di Silvestre, D;Benazzi, L;De Palma, A;Motta, S;Constantin, G;Mauri, P;Laudanna, C;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. T helper 1 effector memory CD4+ T cells protect the skin from poxvirus infection
    Authors: JC Harbour, M Abdelbary, JB Schell, SP Fancher, JJ McLean, TJ Nappi, S Liu, TJ Nice, Z Xia, K Früh, JC Nolz
    Cell Reports, 2023-04-21;42(5):112407.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. A dhesion analysis via a tumor vasculature-like microfluidic device identifies CD8+ T�cells with enhanced tumor homing to improve cell therapy
    Authors: CP Camargo, AK Muhuri, Y Alapan, LF Sestito, M Khosla, MP Manspeaker, AS Smith, CM Paulos, SN Thomas
    Cell Reports, 2023-02-26;42(3):112175.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  5. The effect of type-2 diabetes conditions on neutrophil rolling adhesion
    Authors: K Taverner, Y Murad, AB Yasunaga, C Furrer, J Little, ITS Li
    Bmc Research Notes, 2022-12-03;15(1):355.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Structural characterization and antagonistic effect against P-selectin-mediated function of SFF-32, a fucoidan fraction from Sargassum fusiforme
    Authors: S Wu, J Liu, Y Zhang, J Song, Z Zhang, Y Yang, M Wu, H Tong
    Journal of ethnopharmacology, 2022-06-01;295(0):115408.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  7. A humanized beta2 integrin knockin mouse reveals localized intra- and extravascular neutrophil integrin activation in�vivo
    Authors: L Wen, A Marki, Z Wang, M Orecchioni, J Makings, M Billitti, E Wang, SSA Suthahar, K Kim, WB Kiosses, Z Mikulski, K Ley
    Cell Reports, 2022-05-31;39(9):110876.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Lung-Derived Selectins Enhance Metastatic Behavior of Triple Negative Breast Cancer Cells
    Authors: SU Khan, Y Xia, D Goodale, G Schoettle, AL Allan
    Biomedicines, 2021-10-30;9(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Mitofusin-2 regulates leukocyte adhesion and beta2 integrin activation
    Authors: W Liu, AY Hsu, Y Wang, T Lin, H Sun, JS Pachter, A Groisman, M Imperioli, FW Yungher, L Hu, P Wang, Q Deng, Z Fan
    Journal of leukocyte biology, 2021-09-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Quantification of fast molecular adhesion by fluorescence footprinting
    Authors: AB Yasunaga, ITS Li
    Science Advances, 2021-08-18;7(34):.
    Species: Human
    Sample Types: Recombinant Proteins
    Applications: Bioassay
  11. The vimentin rod domain blocks P-selectin-P-selectin glycoprotein ligand 1 interactions to attenuate leukocyte adhesion to inflamed endothelium
    Authors: FW Lam, CA Brown, C Valladolid, DC Emebo, TG Palzkill, MA Cruz
    PLoS ONE, 2020-10-13;15(10):e0240164.
    Species: N/A
    Sample Types: Complex Sample Type
    Applications: Bioassay
  12. Selectin Binding Sites Are Involved in Cell Adhesive Properties of Head and Neck Squamous Cell Carcinoma
    Authors: U Valentiner, J Knips, R Pries, T Clauditz, A Münscher, G Sauter, B Wollenberg, U Schumacher
    Cancers (Basel), 2019-10-28;11(11):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  13. CAR exosomes derived from effector CAR-T cells have potent antitumour effects and low toxicity
    Authors: W Fu, C Lei, S Liu, Y Cui, C Wang, K Qian, T Li, Y Shen, X Fan, F Lin, M Ding, M Pan, X Ye, Y Yang, S Hu
    Nat Commun, 2019-09-25;10(1):4355.
    Species: Human
    Sample Types: Exosomes
    Applications: ELISA Control
  14. Doxycycline-Dependent Self-Inactivation of CRISPR-Cas9 to Temporally Regulate On- and Off-Target Editing
    Authors: A Kelkar, Y Zhu, T Groth, G Stolfa, AB Stablewski, N Singhi, M Nemeth, S Neelamegha
    Mol. Ther., 2019-09-12;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Frontline Science: A flexible kink in the transmembrane domain impairs β2 integrin extension and cell arrest from rolling
    Authors: H Sun, Z Fan, AR Gingras, MA Lopez-Rami, MH Ginsberg, K Ley
    J. Leukoc. Biol., 2019-09-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Dynamic biochemical tissue analysis detects functional selectin ligands on human cancer tissues
    Authors: EW Martin, R Malgor, VA Resto, DJ Goetz, MM Burdick
    Sci Rep, 2019-06-11;9(1):8511.
    Species: Human
    Sample Types: Protein
    Applications: Bioassay
  17. Central memory CD8+ T cells become CD69+ tissue-residents during viral skin infection independent of CD62L-mediated lymph node surveillance
    Authors: JF Osborn, SJ Hobbs, JL Mooster, TN Khan, AM Kilgore, JC Harbour, JC Nolz
    PLoS Pathog., 2019-03-15;15(3):e1007633.
    Species: Human
    Sample Types: Whole Cells
    Applications: Binding Assay
  18. P-Selectin Glycoprotein Ligand-1 (PSGL-1) Expressing CD4 T Cells Contribute Plaque Instability in Acute Coronary Syndrome
    Authors: K Kitamura, K Sato, M Sawabe, M Yoshida, N Hagiwara
    Circ. J., 2018-06-30;0(0):.
    Species: Human
    Sample Types: Whole Cells
  19. C/EBP? drives interactions between human MAIT cells and endothelial cells that are important for extravasation
    Authors: CH Lee, HH Zhang, SP Singh, L Koo, J Kabat, H Tsang, TP Singh, JM Farber
    Elife, 2018-02-22;7(0):.
    Species: Human
    Sample Types:
    Applications: Bioassay
  20. Recombinant Human Vimentin Binds to P-Selectin and Blocks Neutrophil Capture and Rolling on Platelets and Endothelium
    Authors: FW Lam, Q Da, B Guillory, MA Cruz
    J. Immunol., 2018-01-15;0(0):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Bioassay
  21. P-Selectin and ICAM-1 synergy in mediating THP-1 monocyte adhesion in hemodynamic flow is length dependent
    Authors: EE Edwards, SN Thomas
    Integr Biol (Camb), 2017-04-18;9(4):313-327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Trafficking receptor signatures define blood plasmablasts responding to tissue-specific immune challenge
    Authors: Y Seong, NH Lazarus, L Sutherland, A Habtezion, T Abramson, XS He, HB Greenberg, EC Butcher
    JCI Insight, 2017-03-23;2(6):e90233.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  23. Mapping cell surface adhesion by rotation tracking and adhesion footprinting
    Authors: IT Li, T Ha, YR Chemla
    Sci Rep, 2017-03-14;7(0):44502.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  24. Development of Polymer Microcapsules Functionalized with Fucoidan to Target P-Selectin Overexpressed in Cardiovascular Diseases
    Authors: Bo Li
    Adv Healthc Mater, 2016-12-12;0(0):.
    Applications: Bioassay
  25. Non-fucosylated CB CD34(+) cells represent a good target for enforced fucosylation to improve engraftment following cord blood transplantation
    Authors: Simon N Robinson
    Cytotherapy, 2016-12-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion
    Sci Rep, 2016-07-26;6(0):30392.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  27. Glycopeptide analogues of PSGL-1 inhibit P-selectin in vitro and in vivo.
    Authors: Krishnamurthy V, Sardar M, Ying Y, Song X, Haller C, Dai E, Wang X, Hanjaya-Putra D, Sun L, Morikis V, Simon S, Woods R, Cummings R, Chaikof E
    Nat Commun, 2015-03-31;6(0):6387.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry
  28. Lipid raft-associated beta-adducin is required for PSGL-1-mediated neutrophil rolling on P-selectin.
    Authors: Xu T, Liu W, Yang C, Ba X, Wang X, Jiang Y, Zeng X
    J Leukoc Biol, 2014-11-25;97(2):297-306.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Evidence for a lectin specific for sulfated glycans in the salivary gland of the malaria vector, Anopheles gambiae.
    Authors: Francischetti I, Ma D, Andersen J, Ribeiro J
    PLoS ONE, 2014-09-10;9(9):e107295.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Selectins mediate small cell lung cancer systemic metastasis.
    Authors: Heidemann F, Schildt A, Schmid K, Bruns O, Riecken K, Jung C, Ittrich H, Wicklein D, Reimer R, Fehse B, Heeren J, Luers G, Schumacher U, Heine M
    PLoS ONE, 2014-04-03;9(4):e92327.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  31. IL-15 regulates memory CD8+ T cell O-glycan synthesis and affects trafficking.
    Authors: Nolz J, Harty J
    J Clin Invest, 2014-02-10;124(3):1013-26.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  32. Intravaginal TLR agonists increase local vaccine-specific CD8 T cells and human papillomavirus-associated genital-tumor regression in mice.
    Authors: Domingos-Pereira S, Decrausaz L, Derre L, Bobst M, Romero P, Schiller J, Jichlinski P, Nardelli-Haefliger D
    Mucosal Immunol, 2012-09-12;6(2):393-404.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  33. Low anticoagulant heparin targets multiple sites of inflammation, suppresses heparin-induced thrombocytopenia, and inhibits interaction of RAGE with its ligands.
    Authors: Rao NV, Argyle B, Xu X, Reynolds PR, Walenga JM, Prechel M, Prestwich GD, MacArthur RB, Walters BB, Hoidal JR, Kennedy TP
    Am. J. Physiol., Cell Physiol., 2010-04-07;299(1):C97-110.
    Species: Human
    Sample Types: Protein, Whole Cells
    Applications: Binding Assay, ELISA (Capture)
  34. Cutaneous lymphocyte-associated antigen (CLA) T cells up-regulate P-selectin ligand expression upon their activation.
    Authors: Ni Z, Walcheck B
    Clin. Immunol., 2009-08-08;133(2):257-64.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  35. The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion.
    Authors: Kato N, Yuzawa Y, Kosugi T
    J. Am. Soc. Nephrol., 2009-05-14;20(7):1565-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Binding Assay
  36. Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins.
    Authors: Kerr SC, Fieger CB, Snapp KR, Rosen SD
    J. Immunol., 2008-07-15;181(2):1480-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  37. CD43, but not P-selectin glycoprotein ligand-1, functions as an E-selectin counter-receptor in human pre-B-cell leukemia NALL-1.
    Authors: Nonomura C, Kikuchi J, Kiyokawa N, Ozaki H, Mitsunaga K, Ando H, Kanamori A, Kannagi R, Fujimoto J, Muroi K, Furukawa Y, Nakamura M
    Cancer Res., 2008-02-01;68(3):790-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  38. Kinetic analysis of heparin and glucan sulfates binding to P-selectin and its impact on the general understanding of selectin inhibition.
    Authors: Simonis D, Fritzsche J, Alban S, Bendas G
    Biochemistry, 2007-04-26;46(20):6156-64.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Surface Plasmon Resonance
  39. Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-mediated neutrophil rolling.
    Authors: Bestebroer J, Poppelier MJ, Ulfman LH, Lenting PJ, Denis CV, van Kessel KP, van Strijp JA, de Haas CJ
    Blood, 2007-04-01;109(7):2936-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  40. Chondroitin sulfate glycosaminoglycans as major P-selectin ligands on metastatic breast cancer cell lines.
    Authors: Monzavi-Karbassi B, Stanley JS, Hennings L, Jousheghany F, Artaud C, Shaaf S, Kieber-Emmons T
    Int. J. Cancer, 2007-03-15;120(6):1179-91.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Elevated platelet microparticle levels in nonvalvular atrial fibrillation: relationship to p-selectin and antithrombotic therapy.
    Authors: Choudhury A, Chung I, Blann AD, Lip GY
    Chest, 2007-03-01;131(3):809-15.
    Applications: ELISA (Standard)
  42. Varied levels of reactivity by different E-selectin/Fc constructs with cutaneous lymphocyte-associated antigen (CLA)(+) CD4(+) T cells.
    Authors: Ni</LastName><ForeName>Zh Z</Initial, Ni Z, Walcheck B
    Immunol. Lett., 2007-01-03;108(2):179-82.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  43. The monoclonal antibody CHO-131 identifies a subset of cutaneous lymphocyte-associated antigen T cells enriched in P-selectin-binding cells.
    Authors: Ni Z, Campbell JJ, Niehans G, Walcheck B
    J. Immunol., 2006-10-01;177(7):4742-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  44. The majority of human peripheral blood CD4+CD25highFoxp3+ regulatory T cells bear functional skin-homing receptors.
    Authors: Hirahara K, Liu L, Clark RA, Yamanaka K, Fuhlbrigge RC, Kupper TS
    J. Immunol., 2006-10-01;177(7):4488-94.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  45. P-Selectin-mediated acute inflammation can be blocked by chemically modified heparin, RO-heparin.
    Authors: Gao Y, Li N, Fei R, Chen Z, Zheng S, Zeng X
    Mol. Cells, 2005-06-30;19(3):350-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay, Flow Cytometry

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Reviews for Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF

Average Rating: 4.8 (Based on 4 Reviews)

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Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF
By Anonymous on 03/19/2019
Application: Binding assay/Protein-protein interaction

Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF
By Fuming Zhang on 05/04/2018
Application: Binding assay/Protein-protein interaction

Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF
By Katherine Birmingham on 12/01/2017
Application: Cell Adhesion
Reason for Rating: Great product and customer service was very helpful in assisting in ordering the product in bulk.

Recombinant Human P-Selectin/CD62P Fc Chimera Protein, CF
By Anonymous on 11/03/2016
Application: Cell migration/motility
Reason for Rating: Functions ideally for rolling cell adhesion type of experiments.

Leukocyte rolling adhesion experiment in parallel plate flow chamber