P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L Selectin,P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.
ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals. Furthermore, a number of studies have reported that levels of P-Selectin may be elevated or lowered in subjects with a variety of pathological conditions.Recombinant Human P-Selectin/CD62P Protein, CF
R&D Systems | Catalog # ADP3
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Key Product Details
- R&D Systems CHO-derived Recombinant Human P-Selectin/CD62P Protein (ADP3)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
CHO
Accession Number
Applications
Bioactivity
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Product Specifications
Source
Chinese Hamster Ovary cell line, CHO-derived human P-Selectin/CD62P protein
Trp42-Ala771
Trp42-Ala771
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Trp42
Predicted Molecular Mass
80 kDa
SDS-PAGE
121-124 kDa, reducing conditions
Activity
Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at room temperature.
When 5 x 104 cells/well are added to human sP-Selectin coated plates (10 μg/mL with 100 μL/well), >50% will adhere after 1 hour incubation at room temperature.
Reviewed Applications
Read 1 review rated 5 using ADP3 in the following applications:
Formulation, Preparation, and Storage
ADP3
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS containing Calcium and Magnesium with Sorbitol. |
| Reconstitution | Reconstitute at 1 mg/mL in sterile, deionized water.
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| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Calculators
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
UniProt
Additional P-Selectin/CD62P Products
Product Documents for Recombinant Human P-Selectin/CD62P Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Human P-Selectin/CD62P Protein, CF
For research use only
Citations for Recombinant Human P-Selectin/CD62P Protein, CF
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Application: In vivo studyVerified Customer | Posted 07/29/2016
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