Recombinant Human SLITRK6 Protein, CF

Catalog # Availability Size / Price Qty
8985-SK-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SLITRK6 Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 50 ng on a nitrocellulose-coated microplate.
Source
Mouse myeloma cell line, NS0-derived human SLITRK6 protein
Ile16-Ala604, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ile16 & His19
Predicted Molecular Mass
66 kDa
SDS-PAGE
72-95 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8985-SK

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SLITRK6

SLITRK6 (Slit and Trk-like family member 6) is a type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and (save for SLITRK1) a Trk-like cytoplasmic region. Mature human SLITRK6 consists of a 582 amino acid (aa) extracellular domain (ECD) with 5 leucine rich repeats (LRR) flanked by N-and C-terminal LRR domains, followed by 6 more LRR flanked by another pair of N- and C-terminal LRR domains (1, 2). Within the ECD, human SLITRK6 shares 88% aa sequence identity with mouse and rat SLITRK6. SLITRK6 is expressed in multiple regions of the developing nervous system, notably in the otic vesicle, cochlear and vestibular sensory epithelia, and spiral and vestibular ganglion neurons (3, 4). It can suppress neurite outgrowth and promote the formation of excitatory and inhibitory presynaptic structures (2, 5). Mice lacking SLITRK6 exhibit delayed retinal synaptogenesis and multiple auditory abnormalities as well as defective auditory sensory innervation, neuronal loss in the vestibular ganglia, and reduced cochlear expression of BDNF, NT-3, TrkB, and TrkC (4, 6, 7). In humans, nonsense mutations of SLITRK6 are associated with myopia and sensorial deafness (6).

References
  1. Aruga, J. et al. (2003) Gene 315:87.
  2. Aruga, J. and K. Mikoshiba (2003) Mol. Cell. Neurosci. 24:117.
  3. Aruga, J. (2003) Gene Expr. Patterns 3:727.
  4. Katayama, K.-I. et al. (2009) PLoS ONE 4:e7786.
  5. Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
  6. Tekin, M. et al. (2013) J. Clin. Invest. 123:2094.
  7. Matsumoto, Y. et al. (2011) PLoS ONE 6:e16497.
Long Name
SLIT and NTRK-like Family, Member 6
Entrez Gene IDs
84189 (Human); 239250 (Mouse); 290467 (Rat)
Alternate Names
FLJ22774; MGC119595; MGC119596; MGC119597,4832410J21Rik; SLIT and NTRK-like family, member 6; SLIT and NTRK-like protein 6; slit and trk like gene 6; SLITRK6

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