Recombinant Human SorCS2 Protein, CF

Catalog # Availability Size / Price Qty
4238-SR-050
R&D Systems Recombinant Proteins and Enzymes
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Recombinant Human SorCS2 Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized rhSorCS2 at 1 µg/mL (100 µL/well) can bind rh beta -NGF with an apparent KD < 50 nM.
Source
Mouse myeloma cell line, NS0-derived human SorCS2 protein
Ser70-Gly1078, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ser70
Predicted Molecular Mass
113 kDa
SDS-PAGE
120-140 kDa, reducing conditions

Product Datasheets

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4238-SR

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4238-SR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: SorCS2

SorCS2 is a type I transmembrane glycoprotein receptor that belongs to the mammalian Vps10p (vacuolar protein-sorting 10 protein) family (1, 2). Family receptors include sortilin, SorLA, and three SorCS proteins. All SorCS proteins are predominantly expressed in the brain, especially during development, but vary in other locations (1 - 3). SorCS2 mRNA has also been found in the kidney, lung, testis and heart (1, 2). SorCS2 presumably mediates endocytosis, but its ligands are not well-defined. However, the other SorCS proteins have been shown to bind NGF and PDGF-BB (4 - 6). Human SorCS2 is synthesized as a 1159 amino acid (aa) prepro form with a 50 aa signal sequence and a potential furin-type proteolytic processing site at aa 119. This would produce a mature SorCS2 protein of 1040 aa with a 959 aa extracellular/lumenal domain (ECD), a 21 aa transmembrane domain and a 60 aa cytoplasmic domain. The ECD contains an imperfect leucine-rich repeat (LRR) and a Vps10p domain (2). Within the ECD, human SorCS2 shares 89%, 88%, 88% and 79% aa identity with mouse, rat, equine and canine SorCS2, respectively. It also shares 46% aa identity with the ECD of both SorCS1 and SorCS3. Unlike other SorCS, shedding of the SorCS2 ECD occurs very slowly and is mainly independent of the metalloproteinase TACE/ADAM17 (4).

 

References
  1. Hampe, W. et al. (2001) Hum. Genet. 108:529.
  2. Rezgaoui, M. et al. (2001) Mech. Dev. 100:335.
  3. Hermey, G. et al. (2004) J. Neurochem. 88:1470.
  4. Hermey, G. et al. (2006) Biochem. J. 395:285.
  5. Westergaard, U. et al. (2004) J. Biol. Chem. 279:50221.
  6. Westergaard, U. B. et al. (2005) FEBS Lett. 579:1172.
Long Name
Sortilin-related VPS10 Domain Containing Receptor 2
Entrez Gene IDs
57537 (Human); 81840 (Mouse)
Alternate Names
KIAA1329VPS10 domain receptor protein; SorCS2; sortilin-related VPS10 domain containing receptor 2; VPS10 domain-containing receptor SorCS2

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