Recombinant Human VSIG8 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9200-VS-050
Recombinant Human VSIG8 Fc Chimera Protein Bioactivity
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Recombinant Human VSIG8 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human VSIG8 protein
Human VSIG8
(Val22-Gly263)
Accession # Q5VU13
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Val22
Structure / Form
Disulfide-linked homodimer 
Predicted Molecular Mass
54 kDa
SDS-PAGE
56-64 kDa, reducing conditions

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9200-VS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9200-VS

Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Acetate, Mannitol and Trehalose.
Reconstitution Reconstitute at 200 μg/mL in Water.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Bioactivity Recombinant Human VSIG8 Fc Chimera Protein Bioactivity View Larger

Recombinant Human VSIG8 Fc Chimera (Catalog # 9200-VS) inhibits anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 1-5 μg/mL.

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Background: VSIG8

VSIG8 (V-set and immunoglobulin domain containing 8), also known as C1orf204, is an approximately 45 kDa type I transmembrane protein of the B7 family within the Ig superfamily. Mature human VSIG8 consists of a 242 amino acid (aa) extracellular domain (ECD) containing two V-type Ig-like domains, a 21 aa transmembrane domain, and a 130 aa cytoplasmic domain. Within the ECD, human VSIG8 shares 88% and 89% aa identity with mouse and rat VSIG8, respectively. Alternative splicing generates a long isoform of human VSIG8 with a substitution in the cytoplasmic juxtamembrane region and a 124 aa extension at the C-terminus. VSIG8 was identified from proteomic analysis of human hair shafts (1, 2). It is expressed in the hair follicle and shaft, superficial layers of the nail matrix, and superficial layers of oral epithelium (3). R&D Systems in-house testing indicates that VSIG8 inhibits the production of IL-2 by activated T cells.

References
  1. Rice, R.H. et al. (2010) J. Proteome Res. 9:6752.
  2. Lee, Y.J. et al. (2006) Mol. Cell. Proteomics 5:789.
  3. Rice, R.H. et al. (2011) J. Invest. Dermatol. 131:1936.
Long Name
V-Set and Immunoglobulin Domain Containing 8
Entrez Gene IDs
391123 (Human); 240916 (Mouse); 289236 (Rat); 102123183 (Cynomolgus Monkey)
Alternate Names
V-set and immunoglobulin domain containing 8; VSIG8

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Recombinant Human VSIG8 Fc Chimera Protein, CF
By Anonymous on 03/24/2018
Application: Binding assay/Protein-protein interaction