Recombinant Human VSTM2L HA-tag Protein, CF

R&D Systems | Catalog # 2111-VT

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Key Product Details

  • R&D Systems CHO-derived Recombinant Human VSTM2L HA-tag Protein (2111-VT)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

CHO

Accession Number

Q96N03

Applications

Bioactivity
View all VSTM2L Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human VSTM2L protein
Hemagglutinin Tag
(YPYDVPDYA)
 Human VSTM2L
(Thr25-Leu204) Accession # Q96N03
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

HA-tag followed by Thr25

Predicted Molecular Mass

21 kDa

SDS-PAGE

21-28 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC).
The ED50 for this effect is 0.6-6 μg/mL.

Scientific Data Images for Recombinant Human VSTM2L HA-tag Protein, CF

Recombinant Human VSTM2L HA-tag Protein Bioactivity

Recombinant Human VSTM2L HA-tag Protein Bioactivity

Recombinant Human VSTM2L (Catalog # 2111-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.6-6 μg/mL.
Recombinant Human VSTM2L HA-tag Protein SDS-PAGE

Recombinant Human VSTM2L HA-tag Protein SDS-PAGE

2 μg/lane of Recombinant Human VSTM2L was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 21-28 kDa.

Formulation, Preparation, and Storage

2111-VT
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSTM2L

V-set and transmembrane domain-containing protein 2-like protein (VSTM2L), also known as C20 or f102, is a 22 kDa extracellular protein belonging to the Immunoglobulin superfamily. VSTM2L contains an N-terminal signal peptide (aa 1-25), and an Ig-like domain (aa 41-158) with a disulfide bond between Cys62 and Cys142. VSTM2L is expressed primarily in the central nervous system, but is also detected in serum and cultured cells (1). It has been found to be an antagonist of the neuroprotective peptide Humanin (1). Human VSTM2L has a 94% sequence identity to rat and mouse homolog. VSTM2L functions as an inhibitory receptor in macrophages (2). Our in house data show that VSTM2L inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.

References

  1. Rossini L. et al. (2011) FASEB J. 25:1983.
  2. Yu, Z. et al. (2001) J. Biol. Chem. 276: 23816.

Long Name

V-Set And Transmembrane Domain Containing 2 Like

Alternate Names

C20orf102, Gm691

Entrez Gene IDs

128434 (Human); 277432 (Mouse)

Gene Symbol

VSTM2L

UniProt

Q96N03

Additional VSTM2L Products

Product Documents for Recombinant Human VSTM2L HA-tag Protein, CF

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Product Specific Notices for Recombinant Human VSTM2L HA-tag Protein, CF

For research use only

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