Recombinant Human VSTM2L HA-tag Protein, CF

Catalog # Availability Size / Price Qty
2111-VT-050
Recombinant Human VSTM2L HA-tag Protein, CF Bioactivity
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Recombinant Human VSTM2L HA-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.6-6 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived human VSTM2L protein
Hemagglutinin Tag
(YPYDVPDYA)
Human VSTM2L
(Thr25-Leu204) Accession # Q96N03
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
HA-tag followed by Thr25
Predicted Molecular Mass
21 kDa
SDS-PAGE
21-28 kDa, reducing conditions

Product Datasheets

2111-VT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Recombinant Human VSTM2L HA-tag Protein, CF Bioactivity View Larger

Recombinant Human VSTM2L (Catalog # 2111-VT) inhibits IFN-gamma secretion by humanperipheral blood mononuclear cells in the presence of anti-CD3 antibody. TheED50 for this effect is 0.6-6 μg/mL.

SDS-PAGE Recombinant Human VSTM2L HA-tag Protein, CF SDS-PAGE View Larger

2 μg/lane of Recombinant Human VSTM2L was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 21-28 kDa.

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Background: VSTM2L

V-set and transmembrane domain-containing protein 2-like protein (VSTM2L), also known as C20 or f102, is a 22 kDa extracellular protein belonging to the Immunoglobulin superfamily. VSTM2L contains an N-terminal signal peptide (aa 1-25), and an Ig-like domain (aa 41-158) with a disulfide bond between Cys62 and Cys142. VSTM2L is expressed primarily in the central nervous system, but is also detected in serum and cultured cells (1). It has been found to be an antagonist of the neuroprotective peptide Humanin (1). Human VSTM2L has a 94% sequence identity to rat and mouse homolog. VSTM2L functions as an inhibitory receptor in macrophages (2). Our in house data show that VSTM2L inhibits the human T cell activation, including anti-CD3 induced IL-2 and IFN-gamma secretion, and T cell proliferation.

References
  1. Rossini L. et al. (2011) FASEB J. 25:1983.
  2. Yu, Z. et al. (2001) J. Biol. Chem. 276: 23816.
Long Name
V-Set And Transmembrane Domain Containing 2 Like
Entrez Gene IDs
128434 (Human); 277432 (Mouse)
Alternate Names
C20orf102; chromosome 20 open reading frame 102; dJ1118M15.2; Gm691; V-set and transmembrane domain containing 2 like; V-set and transmembrane domain-containing protein 2-like protein; VSTM2L

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