Recombinant Mouse 5T4 Fc Chimera Protein, CF

R&D Systems | Catalog # 5049-TG

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse 5T4 Fc Chimera Protein (5049-TG)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9Z0L0

Structure / Form

Disulfide-linked homodimer

Applications

Bioactivity
View all 5T4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse 5T4 protein
Mouse 5T4
(Ser35-Ser361)
Accession # Q9Z0L0		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser35

Predicted Molecular Mass

62 kDa

SDS-PAGE

87-96 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse 5T4 Fc Chimera (Catalog# 5049-TG) is immobilized at 2 µg/mL (100 µL/well), Biotinylated Recombinant Human Frizzled-8 Fc Chimera binds with an ED50 of 3-18 μg/mL.

Scientific Data Images for Recombinant Mouse 5T4 Fc Chimera Protein, CF

Recombinant Mouse 5T4 Fc Chimera Protein SDS-PAGE

Recombinant Mouse 5T4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse 5T4 Fc Chimera (Catalog # 5049-TG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 87-96 kDa and 170-190 kDa, respectively.

Formulation, Preparation, and Storage

5049-TG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 1 mg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: 5T4

5T4, also known as Trophoblast glycoprotein and Wnt-activated inhibitory factor 1 (WAIF1), is a single-pass transmembrane protein which may function as an inhibitor of Wnt/beta-catenin signaling (1). Distinct regions on the 5T4 ectodomain which contains eight leucine-rich repeats are thought to antagonize Wnt signaling (2). 5T4 has limited expression in normal adult tissues and in some solid tumors, while expression is elevated in human carcinomas which have been correlated with poor survival and prognosis (3-5). Mouse 5T4 is a 426 amino acid (aa) protein that includes a 31 aa signal peptide, a 330 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 44 aa cytoplasmic tail. Within the ECD, mouse 5T4 shares 78% and 94% aa sequence identity with human and rat 5T4, respectively. It has been identified that TIP-2/GIPC, a common interacting protein involved in cancer, interacts with 5T4 through its PDZ domain (6). 5T4 inhibits Wnt/ beta -catenin signaling by binding to LRP6 and inhibit Wnt-induced LRP6 internalization into endocytic vesicles (1). Conversely, 5T4 can activate noncanonical Wnt signaling through DKK1 (1). In addition to LRP6, in-house data indicates 5T4 can also interact with Frizzled8.

References

  1. Kagermeier-Schenk, B. et al. (2011) Dev. Cell. 21:1129.
  2. Zhao, Y. et al. (2014) Structure. 22:612.
  3. Starzynska, T. et al. (1994) Br. J. Cancer. 69:899.
  4. Naganuma, H. et al. (2002) Anticancer Res. 22:1033.
  5. Harris, J.L. et al. (2018) Int. J. Biochem. Cell Biol. 99:28.
  6. Awan, A. et al. (2002) BBRC 290:1030.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Q9Z0L0

Additional 5T4 Products

Product Documents for Recombinant Mouse 5T4 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Mouse 5T4 Fc Chimera Protein, CF

For research use only

Related Research Areas

Cancer Biomarkers

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