Recombinant Mouse AMIGO3 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9425-AM-050

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Recombinant Mouse AMIGO3 Fc Chimera Protein Bioactivity
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Recombinant Mouse AMIGO3 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse AMIGO3 protein
Mouse AMIGO3
(Thr20-Pro378)
Accession # Q8C2S7
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Thr20
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
67 kDa
SDS-PAGE
78-91 kDa, reducing conditions

Product Datasheets

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9425-AM

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9425-AM

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Bioactivity Recombinant Mouse AMIGO3 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse AMIGO3 Fc Chimera (Catalog # 9425-AM) inhibits IL-2 secretion by mouse T cells in the presence of anti-CD3 antibody. The ED50 for this effect is 0.3-1.5 μg/mL.

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Background: AMIGO3

Amphoterin-Induced Gene and ORF 3 (AMIGO3), also known as Alvin-3, is a member of the AMIGO family of type I transmembrane proteins that contain 6 leucine‑rich repeats (LRRs) and one Ig domain in their extracellular domains. It is ubiquitously expressed and is detected in all tissues studied (1). Mouse AMIGO3 shows less than 40% amino acid sequence homology with AMIGO1 and AMIGO2. The extracellular domain of mouse AMIGO3 shares 90% and 75% amino acid sequence identity with both that of rat and human AMIGO3, respectively. AMIGO family proteins are cell adhesion molecules that exhibit homophilic and heterophilic binding properties and are thought to play roles in neuronal axon tract development and cell adhesion. AMIGO3 mRNA and protein levels are preferentially and significantly raised in DRGN and RGC immediately after central axotomy. Depression of AMIGO3 expression correlates with dorsal column (DC) and optic nerve regeneration. AMIGO3 interacts with NgR1 and p75/TROY forming a functional receptor complex that activates RhoGTP in cells exposed to CNS myelin extracts (CME). AMIGO3 substitutes for LINGO-1 in centrally axotomized DRGN and RGC in the acute phase of injury. AMIGO3-NgR1-p75/TROY receptor complex mediates immediate axon growth inhibitory responses to CNS myelin (2). Our in-house data showed that AMIGO-3 inhibited anti-CD3 induced IL-2 secretion on CD3+ cells, suggesting that AMIGO-3 may be involved in T cell activation.

References
  1. Kuja-Panula J, et al. (2003). J Cell Biol 160:963.
  2. Ahmed Z, et al. (2013) PLoS One. 16:e61878.
Long Name
Amphoterin-induced Gene and ORF 3
Entrez Gene IDs
386724 (Human); 320844 (Mouse)
Alternate Names
adhesion molecule with Ig-like domain 3; ALI3; Alivin-3; AMIGO3; AMIGO-3; amphoterin-induced gene and ORF 3; amphoterin-induced protein 3; KIAA1851; MGC120552

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