Recombinant Mouse Angiopoietin-3 Protein

R&D Systems | Catalog # 738-AN

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Angiopoietin-3 Protein (738-AN)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q9WVH6

Structure / Form

Oligomer

Applications

Binding Activity
View all Angiopoietin-3 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Angiopoietin-3 protein
Mouse Angiopoietin-3
(Gln22 – Ala509)
Accession # Q9WVH6		 IEGR GGGSGGGSGGGS 10-His tag
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

58 kDa

SDS-PAGE

85-90 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmTie-2/Fc Chimera at 4 µg/mL (100 µL/well) can bind rmAngiopoietin-3 with a linear range of 5-100 ng/mL.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 738-AN
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 738-AN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-3

Mouse angiopoietin-3 (ANG-3) (1), is a secreted glycoprotein belonging to the angiopoietin family. It has the characteristic structural motifs of angiopoietins including the coiled-coiled domain near the amino-terminus and a fibrinogen-like domain at the C-terminus. Mouse ANG-3 cDNA encodes a 509 amino acid (aa) precursor protein with a 21 aa signal peptide. It shares 47%, 46% and 54% aa sequence identity with mouse ANG-1, mouse ANG-2 and human ANG-4, respectively. Although the sequence homology is much higher between the human and mouse counterparts for ANG-1 (97%) and ANG-2 (85%), mouse ANG-3 is believed to be an ortholog of human ANG-4 based on chromosomal localization studies (1, 2). Human ANG-4 is highly expressed in lung and in cultured human umbilical vein endothelial cells (HUVECs). In contrast, mouse ANG-3 is expressed in multiple mouse tissues. Human ANG-4 is an agonist that can bind and activate Tie-2, a receptor tyrosine kinase with immunoglobulin and epidermal growth factor homology domains expressed primarily on endothelial cells and early hematopoietic cells (2, 3). Mouse ANG-3 has been reported to be a Tie-2 antagonist. It is likely that mouse ANG-3, like ANG-2, may exert agonist or antagonist activities depending on the cell context (1, 3, 4).

References

  1. Valenzuela, D.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96:1904.
  2. Nishimura, M. et al. (1999) FEBS Lett. 448:254.
  3. Jones, N. et al. (2001) Nat. Rev. Mol. Cell Biol. 2:257.
  4. Teichert-Kuliszewska, K. et al. (2001) Cardiovasc. Res. 49:659.

Alternate Names

ANGPT3

Entrez Gene IDs

11602 (Mouse)

Gene Symbol

ANGPT4

UniProt

Q9WVH6

Additional Angiopoietin-3 Products

Product Documents for Recombinant Mouse Angiopoietin-3 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 738-AN (with carrier)
Download SDS 738-AN/CF (carrier free)

Product Specific Notices for Recombinant Mouse Angiopoietin-3 Protein

For research use only

Citations for Recombinant Mouse Angiopoietin-3 Protein

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