Recombinant Mouse CRELD1 Protein, CF

R&D Systems | Catalog # 4116-CR

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse CRELD1 Protein (4116-CR)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q91XD7

Applications

Bioactivity
View all CRELD1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse CRELD1 protein
Gln30-Glu362, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln30 predicted, sequencing might be blocked

Predicted Molecular Mass

37.1 kDa

SDS-PAGE

50-55 kDa, reducing conditions

Activity

Measured by its ability to induce adhesion of ATDC5 mouse chondrogenic cells.
The ED50 for this effect is typically 0.3-1.5 μg/mL.

Formulation, Preparation, and Storage

4116-CR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CRELD1

Cysteine-rich with EGF-like domain protein 1 (CRELD1) is an approximately 50 kDa transmembrane glycoprotein that contains a 333 amino acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa (1). Within the large ECD, mouse and human CRELD1 share 92% aa sequence identity. CRELD1 is widely expressed during development and in the adult (1). It interacts with Reticulon 3/RTN3 and inhibits the pro-apoptotic activity of RTN3 (2). CRELD1 also associates with Adrenergic alpha‑1 receptors in the prostate and limits their expression (3). Missense mutations of human CRELD1 are associated with susceptibility to atrioventricular septal defects in the heart (4). R&D Systems in-house testing indicates that CRELD1 mediates the adhesion of ATDC-5 cells.

References

  1. Rupp, P.A. et al. (2002) Gene 293:47.
  2. Xiang, R. and S. Zhao (2009) Mol. Cell. Biochem. 331:225.
  3. Nishimune, A. et al. (2010) J. Pharmacol. Sci. 113:169.
  4. Robinson, S.W. et al. (2003) Am. J. Hum. Genet. 72:1047.

Long Name

Cysteine-rich Protein With EGF-like Domains 1

Alternate Names

AVSD2, CIRRIN

Entrez Gene IDs

78987 (Human); 171508 (Mouse); 312638 (Rat)

Gene Symbol

CRELD1

UniProt

Q91XD7

Additional CRELD1 Products

Product Documents for Recombinant Mouse CRELD1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse CRELD1 Protein, CF

For research use only

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