Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

R&D Systems | Catalog # 569-A4

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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Ephrin-A4 Fc Chimera Protein (569-A4)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession Number

Q3UQB5

Structure / Form

Disulfide-linked homodimer

Applications

Binding Activity
View all Ephrin-A4 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Ephrin-A4 protein
Mouse Ephrin-A4
(Leu26 - Gly176)
Accession # Q3UQB5		 DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu26

Predicted Molecular Mass

43.6 kDa (monomer)

SDS-PAGE

50-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Immobilized rmEphA7/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A4/Fc Chimera with a linear range of 0.16-10 ng/mL.

Formulation, Preparation, and Storage

569-A4
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution

Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Ephrin-A4

Ephrin-A4, also known as LERK-4 and EFL-4 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A4 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1 (2, 3). The extracellular domains of human and mouse Ephrin-A4 share 80% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

References

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Alternate Names

EFL-4, EFNA4, EphrinA4, LERK-4

Entrez Gene IDs

1945 (Human); 13639 (Mouse)

Gene Symbol

EFNA4

UniProt

Q3UQB5

Additional Ephrin-A4 Products

Product Documents for Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

For research use only

Related Research Areas

Ephrin Ligands and Eph Receptors

Citations for Recombinant Mouse Ephrin-A4 Fc Chimera Protein, CF

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