Recombinant Mouse IgG2A Fc Protein, CF

Catalog # Availability Size / Price Qty
4460-MG-100

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Citations (3)
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Recombinant Mouse IgG2A Fc Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Bioassay data are not available.
Source
Mouse myeloma cell line, NS0-derived mouse IgG2A protein
Glu98-Lys330, with an N-terminal Met-Asp-Pro
Accession #
N-terminal Sequence
Analysis
Met
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
26.7 kDa (monomer)
SDS-PAGE
34-35 kDa, reducing conditions

Product Datasheets

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4460-MG

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4460-MG

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution It is recommended that sterile PBS be added to the vial to prepare a working stock solution of no less than 100 μg/mL.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: IgG2A

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name
Immunoglobulin G2A Fc
Alternate Names
IgG2A

Citations for Recombinant Mouse IgG2A Fc Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. GITR/GITRL reverse signalling modulates the proliferation of hepatic progenitor cells by recruiting ANXA2 to phosphorylate ERK1/2 and Akt
    Authors: Y He, Y Pei, K Liu, L Liu, Y Tian, H Li, M Cong, T Liu, H Ma, H You, J Jia, D Zhang, P Wang
    Cell Death & Disease, 2022-04-04;13(4):297.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. Invariant NKT Cell Activation Is Potentiated by Homotypic trans-Ly108 Interactions
    Authors: Y Baglaenko, M Cruz Tleug, E Gracey, N Talaei, KP Manion, NH Chang, DM Ferri, T Mallevaey, JE Wither
    J. Immunol., 2017-04-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Differential but competitive binding of Nogo protein and class i major histocompatibility complex (MHCI) to the PIR-B ectodomain provides an inhibition of cells.
    Authors: Matsushita H, Endo S, Kobayashi E, Sakamoto Y, Kobayashi K, Kitaguchi K, Kuroki K, Soderhall A, Maenaka K, Nakamura A, Strittmatter SM, Takai T
    J. Biol. Chem., 2011-06-02;286(29):25739-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Control

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Reviews for Recombinant Mouse IgG2A Fc Protein, CF

Average Rating: 4 (Based on 2 Reviews)

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Recombinant Mouse IgG2A Fc Protein, CF
By Anonymous on 08/18/2021
Application: Binding assay/Protein-protein interaction

Recombinant Mouse IgG2A Fc Protein, CF
By Anonymous on 10/13/2018
Application: CellProlif