Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF

N-Terminal/MPF
Catalog # Availability Size / Price Qty
10516-MS-050
Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein Binding Activity
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Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Osteopontin/OPN  (Catalog # 441-OP) is immobilizedat 0.5 µg/mL,100  μL/well, Recombinant Mouse Mesothelin N‑Terminal (aa 36-288) (Catalog # 10516-MS) binds with an ED50 of 2-12 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Mesothelin protein
Gln36-Leu288, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Gln36 inferred from enzymatic pyroglutamate treatment revealing Thr37
Structure / Form
Monomer
Predicted Molecular Mass
29 kDa
SDS-PAGE
29-45 kDa, under reducing conditions

Product Datasheets

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10516-MS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10516-MS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Scientific Data

Binding Activity Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein Binding Activity View Larger

When Recombinant Mouse Osteopontin/OPN Protein (441-OP) is immobilized at 0.5 μg/mL, 100 μL/well, it binds Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein (10516-MS) with an ED50 of 2-12 ng/mL.

SDS-PAGE Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse Mesothelin (aa 36-288) His-tag Protein (Catalog # 10516-MS) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 29-45 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: Mesothelin

Mesothelin N-terminal, commonly known as Megakaryocyte Potentiating Factor (MPF), is a 31 kDa soluble protein that was originally identified in an PHC-Y5 culture system where it exhibited megakaryocyte potentiating activity (1). MPF is closely related to mesothelin (MSLN), a 40 kDa glycosylphosphatidyl inositol (GPI)-linked protein that is highly overexpressed in various types of cancer, such as mesothelioma, ovarian and pancreatic cancers (2). Both MPF and MSLN originate from a common 71 kDa precursor protein that harbors a furin cleavage site between amino acid residues 288-293. The 31 kDa soluble N-terminal MPF fragment is secreted into blood and the C-terminal 40 kDa fragment remains membrane-bound and classified as mature mesothelin (3). Mouse MPF is composed of residues 36-288 from the precursor protein and contains one N-linked glycosylation site (N93) and one phosphorylated serine (S202). Mouse MPF shares 56% and 84% sequence identity with human and rat MPF, respectively. It is expressed mainly in the heart and lungs (4) and can be upregulated in the presence of Wnt-1 (5). MSLN was shown to be capable of binding to cancer antigen 125/mucin16 (CA125/MUC16). Apart from its role as a megakaryocyte potentiating cytokine, MPF may have the potential to serve as a biomarker for detection and monitoring of malignant mesothelioma and related cancers (6).

References
  1. Yamaguchi, N. et al. (1994) J. Biol. Chem. 260: 22:805.
  2. Hassan R. et al. (2004) Clin. Cancer Res. 10:3937.
  3. Shiomi, K. et al. (2006) Cancer. Sci. 97:928.
  4. Bera T.K. Pastan I. (2000) Mol. Cell. Biol. 20:2902.
  5. Prieve M.G. Moon R.T. (2003) BMC Dev Biol. 3:2.
  6. Raiko, I. et al. (2017) Biochem. Biophy. Res. Com. 486:526.
Entrez Gene IDs
10232 (Human); 56047 (Mouse); 60333 (Rat); 102130460 (Cynomolgus Monkey)
Alternate Names
CAK1 antigen; CAK1; megakaryocyte potentiating factor; Mesothelin; MPF; MPFSMRP; MSLN; Pre-pro-megakaryocyte-potentiating factor; SMR; soluble MPF mesothelin related protein

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