Prolyl Oligopeptidase, also known as prolyl endopeptidase and post-proline cleaving enzyme, is a serine peptidase displaying specificity for the cleavage of Pro-Xaa bonds of oligopeptide substrates (1, 2). The peptidase is known to hydrolyze a variety of biologically active peptides such as bradykinin, substance P, neurotensin, and vasopressin (3). Because of its action on neuropeptides, Prolyl Oligopeptidase is considered to be involved in processes such as learning, memory, and depression (4).
Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
R&D Systems | Catalog # 6339-SE
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Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse Prolyl Oligopeptidase/PREP Protein (6339-SE)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession Number
Applications
Enzyme Activity
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Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Prolyl Oligopeptidase/PREP protein
Leu2-Gln710, with an N-terminal Met and 6-His tag
Accession # Q9QUR6
Leu2-Gln710, with an N-terminal Met and 6-His tag
Accession # Q9QUR6
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
82 kDa
SDS-PAGE
68-73 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC).
The specific activity is >4,500 pmol/min/μg, as measured under the described conditions.
The specific activity is >4,500 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation, and Storage
6339-SE
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and Glycerol. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Prolyl Oligopeptidase/PREP
References
- Szeltner, Z and L. Polgár (2008) Current Protein Pept. Sci. 9:96.
- Yoshimoto, T. et al. (1977) Biochemistry. 16:2942.
- Wilk, S. (1983) Life Sci. 33:2149.
- Männisto, P.T. et al. (2007) Drug News Perspect. 20:293.
Alternate Names
PE, PREP, rPop
Gene Symbol
PREP
UniProt
Additional Prolyl Oligopeptidase/PREP Products
Product Documents for Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF
Coomassie is a registered trademark of Imperial Chemical Industries Ltd.
For research use only
Related Research Areas
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Protocols
View specific protocols for Recombinant Mouse Prolyl Oligopeptidase/PREP Protein, CF (6339-SE):
Materials
- Assay Buffer: 25 mM Tris, 250 mM NaCl, 2.5 mM DTT, pH 7.5
- Recombinant Mouse Prolyl Oligopeptidase/PREP (rmPREP) (Catalog # 6339-SE)
- Substrate: Z-GP-AMC (Bachem, Catalog # I-1145), 10 mM stock in DMSO
- F16 Black Maxisorp Plate (Nunc, Catalog # 475515)
- Fluorescent Plate Reader (Model: SpectraMax Gemini EM by Molecular Devices) or equivalent
- Dilute rmPREP to 0.1 ng/μL in Assay Buffer.
- Dilute Substrate to 100 μM in Assay Buffer.
- Load into a plate 50 μL of 0.1 ng/μL rmPREP, and start the reaction by adding 50 μL of 100 μM Substrate.
- For Substrate Blanks, load 50 μL of Assay Buffer and 50 μL of 100 μM Substrate.
- Read plate at excitation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes.
- Calculate specific activity:
|
Specific Activity (pmol/min/µg) = |
Adjusted Vmax* (RFU/min) x Conversion Factor** (pmol/RFU) |
| amount of enzyme (µg) |
*Adjusted for Substrate Blank
**Derived using calibration standard 7-Amino, 4-Methyl Coumarin (Sigma, Catalog # A-9891).
- rmPREP: 0.005 μg
- Substrate: 50 μM
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