Recombinant Mouse SNED1 Protein, CF Summary
- R&D Systems CHO-derived Recombinant Mouse SNED1 Protein (9335-SN)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Arg23-Lys1403, with a C-terminal 6-His tag
Analysis
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9335-SN
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Scientific Data
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Immobilized Recombinant Mouse SNED1 (Catalog # 9335-SN) supports the adhesion of CCD-1070Sk human normal skin fibroblasts The ED50 for this effect is 0.1-0.4 μg/mL.
Reconstitution Calculator
Background: SNED1
SNED1 (Sushi, nidogen and EGF-like domain-containing protein 1), also known as stromal nidogen extracellular matrix protein (Snep) and secreted protein SST-3, is an approximately 150 kDa protein (1). Mature mouse SNED1 contains 1379 aa that includes a Sushi/CCP (complement control protein) domain, an NIDO domain, 15 EGF-like domains, and 3 fibronectin type-III domains (1). Mouse SNED1 shares 84% and 96% aa sequence identity with human and rat SNED1, respectively. Alternative splicing generates an isoform lacking the Sushi/CCP domain, normally found in the center of the protein. SNED1 is mainly expressed in kidney stromal cells, but can be found in mesenchymal cells of other embryonic tissues, and within the nervous system (1,2). SNED1 has been found to have a role in tumor progression and metastasis. Studies show that SNED1 is up-regulated in highly metastatic mammary tumors while it is found to have relatively lower expression levels in poorly metastatic mammary tumors (3). SNED1 has also been shown to be up-regulated in pancreatic ductal adenocarcinoma cells which correlates with an increased chemoresistance (4). Additionally, SNED1 is found to be expressed specifically in stroma cells of the kidney and other organs in areas that undergo apoptosis during embryonic development making it important for kidney development (1).
- Leimeister, C. et al. (2004) Developmental Dynamics. 230:371.
- Ueno, H. et al. (2003) Nature Immunology. 4:457.
- Naba, A. et al. (2014) eLife. 3:e01308.
- Longati, P. et al. (2013) BMC Cancer. 13:95.
Citations for Recombinant Mouse SNED1 Protein, CF
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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The novel ECM protein SNED1 mediates cell adhesion via the RGD-binding integrins ?5?1 and ?v?3
Authors: Pally, D;Kapoor, N;Naba, A;
Journal of cell science
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay -
The novel ECM protein SNED1 mediates cell adhesion via ?5?1 integrin
Authors: Pally, D;Kapoor, N;Naba, A;
bioRxiv : the preprint server for biology
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
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