Recombinant Mouse VSIG1 Fc Chimera Protein, CF Summary
Accession # NP_084457
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSIG1 Fc Chimera (Catalog # 9645‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.
VSIG1 (V-set and immunoglobulin domain-containing protein 1), also known as Glycoprotein A34, is a member of the JAM (junctional adhesion molecule) family of proteins (1, 2). It is predominantly expressed in stomach and testis, and was also detected in gastric, esophageal and ovarian cancers (2). VSIG1 is required for the proper differentiation of glandular gastric epithelia, and also plays a role as tumor suppressor (3, 4). This type I transmembrane glycoprotein consists of a 22 amino acid (aa) signal peptide, a 212 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 152 aa cytoplasmic domain. Within the ECD, mouse VSIG1 shares 81% and 94% amino acid sequence identity with human and rat VSIG1, respectively. VSIG1 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG1 inhibits T cell activation, including IL-17 and interferon gamma production.
- Kim, E. et al. (2010) Mol. Cells. 30:443.
- Scanlan, M.J. et al. (2006) Cancer Immun. 6:2.
- Oidovasambuu, O. (2011) PLoS One 10:e25908.
- Inoue, Y. et al. (2017) Cancer Sci. 8:1701.
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