Recombinant Mouse VSIG1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9645-VS-050
Recombinant Mouse VSIG1 Fc Chimera Protein Bioactivity
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Recombinant Mouse VSIG1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-6 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse VSIG1 protein
Mouse VSIG1
(Val23-Glu234)
Accession # NP_084457
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Val23
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
50 kDa
SDS-PAGE
63-76 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9645-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse VSIG1 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse VSIG1 Fc Chimera (Catalog # 9645‑VS) inhibits IL-17 secretion by human peripheral blood mononuclear cells (PBMC), in the presence of anti-CD3 antibody. The ED50 for this effect is 1-6 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG1

VSIG1 (V-set and immunoglobulin domain-containing protein 1), also known as Glycoprotein A34, is a member of the JAM (junctional adhesion molecule) family of proteins (1, 2). It is predominantly expressed in stomach and testis, and was also detected in gastric, esophageal and ovarian cancers (2). VSIG1 is required for the proper differentiation of glandular gastric epithelia, and also plays a role as tumor suppressor (3, 4). This type I transmembrane glycoprotein consists of a 22 amino acid (aa) signal peptide, a 212 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 152 aa cytoplasmic domain. Within the ECD, mouse VSIG1 shares 81% and 94% amino acid sequence identity with human and rat VSIG1, respectively. VSIG1 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG1 inhibits T cell activation, including IL-17 and interferon gamma production.

References
  1. Kim, E. et al. (2010) Mol. Cells. 30:443.
  2. Scanlan, M.J. et al. (2006) Cancer Immun. 6:2.
  3. Oidovasambuu, O. (2011) PLoS One 10:e25908.
  4. Inoue, Y. et al. (2017) Cancer Sci. 8:1701.
Long Name
V-Set and Immunoglobulin Domain Containing 1
Entrez Gene IDs
340547 (Human); 78789 (Mouse); 315920 (Rat); 102123142 (Cynomolgus Monkey)
Alternate Names
Cell surface A33 antigen; dJ889N15.1; Glycoprotein A34; GPA34; MGC44287,1700062D20Rik; V-set and immunoglobulin domain containing 1; V-set and immunoglobulin domain-containing protein 1; VSIG1

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