Recombinant Mouse VSIG10 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
9604-VS-050
Recombinant Mouse VSIG10 Fc Chimera Protein Bioactivity
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Recombinant Mouse VSIG10 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.15-0.9 μg/mL.
Source
Mouse myeloma cell line, NS0-derived mouse VSIG10 protein
Mouse VSIG10
(Leu25-Asn426)
Accession # D3YX43
IEGRMDP Mouse IgG2a
(Glu98-Lys330)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Leu25
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
71 kDa
SDS-PAGE
97-118 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9604-VS

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Image

Bioactivity Recombinant Mouse VSIG10 Fc Chimera Protein Bioactivity View Larger

Recombinant Mouse VSIG10 Fc Chimera (Catalog # 9604-VS) inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC. The ED50 for this effect is 0.15-0.9 μg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSIG10

VSIG10 (V-set and immunoglobulin domain-containing protein 10) is a single-pass type I membrane protein of the Ig superfamily. Mouse VSIG10 cDNA encodes a 558 amino acid (aa) precursor that contains a 20 aa signal sequence, a 406 aa extracellular domain (ECD), a 21 aa transmembrane sequence, and a 111 aa cytoplasmic domain (1). The ECD of mouse VSIG10 contains four Ig-like C2-type domains and shares 63% and 84% aa identity with human and rat VSIG10, respectively. The specific biological function of VSIG10L has yet to be elucidated, but there is evidence to suggest the molecule is involved in cell-cell adhesion. Ig‑like C2-type domains are similar to antibody constant domains and can be found in T cell adhesion molecules, such as Cluster of Differentiation 2 (CD2) along with vascular and intercellular cell adhesion molecules (VCAM and ICAM, respectively) (2). VSIG10 is structurally related to the B7 family of immune regulatory proteins. Our studies at R&D Systems show that VSIG10 inhibits T cell activation, including IL-17 and Interferon gamma production.

References
  1. SwissProt Accession # D3YX43.
  2. Yang H. and E. L. Reinherz (2001) J. Biol. Chem. 276:18775.
Long Name
V-Set and Immunoglobulin Domain Containing 10
Entrez Gene IDs
54621 (Human); 231668 (Mouse); 304529 (Rat)
Alternate Names
FLJ20674; V-set and immunoglobulin domain containing 10; V-set and immunoglobulin domain-containing protein 10; VSIG10

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