Recombinant Mouse VSTM2B Fc Chimera Protein, CF Summary
Accession # Q9JME9
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 200 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSTM2B Fc Chimera (Catalog # 10076-VT) inhibits IFN-gamma secretion by human peripheral blood mononuclear cells in the presence of anti-CD3 antibody. The ED50 for this effect is 2-10 μg/mL.
2 μg/lane of Recombinant Mouse VSTM2B Fc Chimera was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 61-70 kDa and 120-140 kDa, respectively.
V-set and transmembrane domain-containing protein 2B, also known as VSTM-2B, is a single pass Type I membrane protein. Mouse VSTM-2B contains an N-Terminal signal peptide (1-28 aa), an extracellular domain (aa 29-263) which contains a single Ig-like V-Type domain (aa 29-143) with a single disulfide bond between C49 and C127, a helical transmembrane domain (aa 264-284) and a single histidine in the cytoplasm (aa 285). VSTM2B is expressed in multiple organs, with the highest expression in the pyloric antrum. The extracellular domain shares 89% and 96% sequence identity for the human and rat homolog, respectively. Currently, both the expression pattern and biological functions of VSTM2B remain unknown. our in house data show that VSTM2B inhibits anti-CD3 induced IL-2 and IFN-g secretion on human T cells.
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