Recombinant Mouse VSTM2L HA-tag Protein, CF

Catalog # Availability Size / Price Qty
2152-VT-050
Recombinant Mouse VSTM2L HA-tag Protein, CF Bioactivity
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Recombinant Mouse VSTM2L HA-tag Protein, CF Summary

Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.5-3 μg/mL.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse VSTM2L protein
Hemagglutinin Tag (YPYDVPDYA)GlnMouse VSTM2L
(Arg26-Leu202)
N-terminusC-terminus
Accession #
N-terminal Sequence
Analysis
Tyr
Structure / Form
Monomer
Predicted Molecular Mass
21 kDa
SDS-PAGE
19-25 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

2152-VT

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity Bioactivity View Larger

Recombinant Mouse VSTM2L (Catalog # 2152-VT) inhibits anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.5‑3 μg/mL.

SDS-PAGE SDS-PAGE View Larger

2 μg/lane of Recombinant Mouse VSTM2L was resolved with SDS-PAGE underreducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Bluestaining, showing bands at 19-25 kDa.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VSTM2L

V-set and transmembrane domain-containing protein 2-like protein (VSTM2L), also known as C20orf102, is a 22 kDa extracellular protein belonging to the Immunoglobulin superfamily. VSTM2L contains an N-terminal signal peptide (aa 1-25), and an Ig-like domain (aa 41-158) with a disulfide bond between Cys 62 and Cys142. VSTM2L is expressed primarily in the central nervous system, but is also detected in serum and secreted in cultured cells (1). It has been found to be an antagonist of the neuroprotective peptide Humanin (1). Human VSTM2L has a 94% sequence identity to mouse homolog. VSTM2L functions as an inhibitory receptor in macrophages (2). Our in house data show that VSTM2L inhibits human T cell activation, including anti-CD3 induced IFN-gamma secretion.

References
  1. Rossini L. et al. (2011) FASEB J. 25:1983.
  2. Yu, Z. et al. (2001) J. Biol. Chem. 276:23816.
Long Name
V-Set And Transmembrane Domain Containing 2 Like
Entrez Gene IDs
128434 (Human); 277432 (Mouse)
Alternate Names
C20orf102; chromosome 20 open reading frame 102; dJ1118M15.2; Gm691; V-set and transmembrane domain containing 2 like; V-set and transmembrane domain-containing protein 2-like protein; VSTM2L

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