Recombinant Mouse VSTM2L HA-tag Protein, CF Summary
|Hemagglutinin Tag (YPYDVPDYA)||Gln||Mouse VSTM2L|
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Recombinant Mouse VSTM2L (Catalog # 2152-VT) inhibits anti-CD3 antibody induced IFN-gamma secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 0.5-3 μg/mL.
2 μg/lane of Recombinant Mouse VSTM2L was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 19-25 kDa.
V-set and transmembrane domain-containing protein 2-like protein (VSTM2L), also known as C20orf102, is a 22 kDa extracellular protein belonging to the Immunoglobulin superfamily. VSTM2L contains an N-terminal signal peptide (aa 1-25), and an Ig-like domain (aa 41-158) with a disulfide bond between Cys 62 and Cys142. VSTM2L is expressed primarily in the central nervous system, but is also detected in serum and secreted in cultured cells (1). It has been found to be an antagonist of the neuroprotective peptide Humanin (1). Human VSTM2L has a 94% sequence identity to mouse homolog. VSTM2L functions as an inhibitory receptor in macrophages (2). Our in house data show that VSTM2L inhibits human T cell activation, including anti-CD3 induced IFN-gamma secretion.
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